7SSJ

Crystal structure of the DesK-DesR complex in the phosphatase state

Replaces:  5IUN

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D	Sensor histidine kinase DesK	polymer	224	25534.4	3	UniProt (O34757) Pfam (PF07730) Pfam (PF02518) In PDB	Bacillus subtilis
2	B, E, F	Transcriptional regulatory protein DesR	polymer	139	15108.4	3	UniProt (O34723) Pfam (PF00072) In PDB	Bacillus subtilis
3	A, C, D	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	3	Chemie (ACP)
4	A, B, C, D, E...	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
5	B, E, F	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	3	Chemie (BEF)
6	E	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7		water	water		18.0	22	Chemie (HOH)

Sequence modifications

A, C, D: 150 - 370 (UniProt: O34757)

PDB	External Database	Details
Gly 147	-	expression tag
Ser 148	-	expression tag
Gly 149	-	expression tag
Ile 150	Ser 150	engineered mutation
Leu 153	Ser 153	engineered mutation
Ile 157	Arg 157	engineered mutation

B, E, F: 1 - 135 (UniProt: O34723)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	121928.2
Non-Polymers*	Number of molecules	13
	Total formula weight	1955.5
All*	Total formula weight	123883.8

*Water molecules are not included.