7SSI

CRYSTAL STRUCTURE OF THE DESK:DESR-Q10A COMPLEX IN THE PHOSPHOTRANSFER STATE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, E (A, B, D, E)	Sensor histidine kinase DesK	polymer	218	24956.6	4	UniProt (O34757) Pfam (PF07730) Pfam (PF02518)	Bacillus subtilis
2	C, F (C, F)	Transcriptional regulatory protein DesR	polymer	139	15051.3	2	UniProt (O34723) Pfam (PF00072)	Bacillus subtilis
3	G, I, L, N (A, B, D, E)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	4	Chemie (ACP)
4	H, J, K, M, O... (A, B, C, D, E...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
5	Q, R, S, T, U (B, C, D, E, F)	water	water		18.0	10	Chemie (HOH)

Sequence modifications

A, B, D, E: 154 - 370 (UniProt: O34757)

PDB	External Database	Details
Gly 153	-	expression tag
Glu 188	His 188	engineered mutation

C, F: 1 - 135 (UniProt: O34723)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 10	Gln 10	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	129929.0
Non-Polymers*	Number of molecules	10
	Total formula weight	2166.7
All*	Total formula weight	132095.7

*Water molecules are not included.