7SOG

Thermostable actophorin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Actophorin	polymer	135	15183.1	2	UniProt (P37167) Pfam (PF00241)	Acanthamoeba castellanii (Amoeba)
2	C, D, E, F (A, B)	NICKEL (II) ION	non-polymer		58.7	4	Chemie (NI)
3	G, H (A, B)	water	water		18.0	185	Chemie (HOH)

Sequence modifications

A, B: 2 - 135 (UniProt: P37167)

PDB	External Database	Details
Gly 1	-	expression tag
Pro 2	Ser 2	engineered mutation
Lys 21	His 21	engineered mutation
Lys 22	Gln 22	engineered mutation
Ile 27	Thr 27	engineered mutation
Leu 30	Met 30	engineered mutation
Asp 32	Ala 32	engineered mutation
Asp 33	Ser 33	engineered mutation
Lys 41	His 41	engineered mutation
Leu 53	Lys 53	engineered mutation
Ala 54	Ser 54	engineered mutation
Asn 59	Arg 59	engineered mutation
Glu 71	Gln 71	engineered mutation
Val 81	Thr 81	engineered mutation
Ala 99	Thr 99	engineered mutation
Ser 101	Thr 101	engineered mutation
Ala 104	Ser 104	engineered mutation
Asp 110	Val 110	engineered mutation
Asp 122	Ala 122	engineered mutation
Lys 130	Ser 130	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	30366.3
Non-Polymers*	Number of molecules	4
	Total formula weight	234.8
All*	Total formula weight	30601.1

*Water molecules are not included.