7SNW
1.80A Resolution Structure of NanoLuc Luciferase with Bound Inhibitor PC 16026576
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C | Oplophorus-luciferin 2-monooxygenase catalytic subunit | polymer | 174 | 19429.2 | 3 | UniProt (Q9GV45) In PDB | Oplophorus gracilirostris (Luminous shrimp) | 19kOLase |
2 | A, B, C | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
3 | A, B, C | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
4 | A, B, C | 2-(methoxycarbonyl)thiophene-3-sulfonic acid | non-polymer | 222.2 | 3 | Chemie (9YR) | |||
5 | A, B, C | N-[(4-methylphenyl)methyl]-N~2~-phenylglycinamide | non-polymer | 254.3 | 3 | Chemie (9Y4) | |||
6 | A, B | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
7 | A, B | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
8 | water | water | 18.0 | 504 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 169 (UniProt: Q9GV45)
PDB | External Database | Details |
---|---|---|
Ser -4 | - | expression tag |
Asp -3 | - | expression tag |
Asn -2 | - | expression tag |
Met -1 | - | expression tag |
Val 0 | - | expression tag |
Glu 4 | Ala 31 | engineered mutation |
Arg 11 | Gln 38 | engineered mutation |
Leu 18 | Gln 45 | engineered mutation |
Val 27 | Leu 54 | engineered mutation |
Asn 33 | Ala 60 | engineered mutation |
Arg 43 | Lys 70 | engineered mutation |
Ile 44 | Val 71 | engineered mutation |
Ile 54 | Ala 81 | engineered mutation |
Asp 68 | Phe 95 | engineered mutation |
Gln 72 | Leu 99 | engineered mutation |
Lys 75 | Met 102 | engineered mutation |
Val 90 | Ile 117 | engineered mutation |
Glu 115 | Pro 142 | engineered mutation |
Lys 124 | Gln 151 | engineered mutation |
Ile 138 | Tyr 165 | engineered mutation |
Arg 166 | Asn 193 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 58287.5 | |
Non-Polymers* | Number of molecules | 20 |
Total formula weight | 2365.6 | |
All* | Total formula weight | 60653.1 |