7SNR
2.00A Resolution Structure of NanoLuc Luciferase
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Oplophorus-luciferin 2-monooxygenase catalytic subunit | polymer | 174 | 19569.8 | 2 | UniProt (Q9GV45) In PDB | Oplophorus gracilirostris (Luminous shrimp) | 19kOLase |
2 | A | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
3 | A | CITRATE ANION | non-polymer | 189.1 | 1 | Chemie (FLC) | |||
4 | water | water | 18.0 | 151 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 169 (UniProt: Q9GV45)
PDB | External Database | Details |
---|---|---|
Ser -4 | - | expression tag |
Asp -3 | - | expression tag |
Asn -2 | - | expression tag |
Mse -1 | - | expression tag |
Val 0 | - | expression tag |
Glu 4 | Ala 31 | engineered mutation |
Arg 11 | Gln 38 | engineered mutation |
Leu 18 | Gln 45 | engineered mutation |
Val 27 | Leu 54 | engineered mutation |
Asn 33 | Ala 60 | engineered mutation |
Arg 43 | Lys 70 | engineered mutation |
Ile 44 | Val 71 | engineered mutation |
Ile 54 | Ala 81 | engineered mutation |
Asp 68 | Phe 95 | engineered mutation |
Gln 72 | Leu 99 | engineered mutation |
Lys 75 | Met 102 | engineered mutation |
Val 90 | Ile 117 | engineered mutation |
Glu 115 | Pro 142 | engineered mutation |
Lys 124 | Gln 151 | engineered mutation |
Ile 138 | Tyr 165 | engineered mutation |
Arg 166 | Asn 193 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 39139.7 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 307.2 | |
All* | Total formula weight | 39446.9 |