7SJA

Undecorated 13pf E254N microtubule from recombinant human tubulin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, J, K...	Tubulin alpha-1B chain	polymer	457	51018.3	6	UniProt (P68363) Pfam (PF00091) Pfam (PF03953) In PDB	Homo sapiens (Human)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, D, F, G, H...	Tubulin beta-3 chain	polymer	456	51276.4	6	UniProt (Q13509) Pfam (PF00091) Pfam (PF03953) In PDB	Homo sapiens (Human)	Tubulin beta-4 chain,Tubulin beta-III
3	H, I, J, K, L...	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	12	Chemie (GTP)
4	H, I, J, K, L...	MAGNESIUM ION	non-polymer		24.3	12	Chemie (MG)

Sequence modifications

A, C, E, J, K, L: 1 - 451 (UniProt: P68363)

PDB	External Database	Details
His 37	-	insertion
His 37	-	insertion
His 37	-	insertion
His 37	-	insertion
His 37	-	insertion
His 37	-	insertion
Asn 254	Glu 254	engineered mutation

B, D, F, G, H, I: 1 - 450 (UniProt: Q13509)

PDB	External Database	Details
Glu 451	-	expression tag
Asn 452	-	expression tag
Leu 453	-	expression tag
Tyr 454	-	expression tag
Phe 455	-	expression tag
Gln 456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	613768.1
Non-Polymers*	Number of molecules	24
	Total formula weight	6569.8
All*	Total formula weight	620337.9

*Water molecules are not included.