7SJ6

T4 Lysozyme L99A/M102H with 1,2-Azaborine bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Lysozyme	polymer	187	21404.4	2	UniProt (P00720) Pfam (PF00959) In PDB	Enterobacteria phage T4 (Bacteriophage T4)	Lysis protein,Endolysin,Muramidase
2	A, B	1,2-dihydro-1,2-azaborinine	non-polymer		78.9	2	Chemie (B20)
3	A, B	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
4	A, B	BETA-MERCAPTOETHANOL	non-polymer		78.1	2	Chemie (BME)
5	A	2-HYDROXYETHYL DISULFIDE	non-polymer		154.3	1	Chemie (HED)
6	B	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
7		water	water		18.0	499	Chemie (HOH)

Sequence modifications

A, B: 1 - 164 (UniProt: P00720)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 12	Arg 12	conflict
Cys 21	Thr 21	conflict
Asp 38	Ser 38	conflict
Ala 99	Leu 99	engineered mutation
His 102	Met 102	engineered mutation
Val 108	Glu 108	conflict
Val 117	Ser 117	conflict
Arg 137	Ile 137	conflict
Cys 142	Thr 142	conflict
Asp 144	Asn 144	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	42808.9
Non-Polymers*	Number of molecules	11
	Total formula weight	1007.7
All*	Total formula weight	43816.5

*Water molecules are not included.