7SGC

Replication Initiator Protein REPE54 and cognate DNA sequence with terminal five prime phosphates.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (5'-D(P*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*G)-3')	polymer	21	6383.1	1		Escherichia coli
2	B (B)	DNA (5'-D(P*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*G)-3')	polymer	21	6503.2	1		Escherichia coli
3	C (C)	RepB family plasmid replication initiator protein	polymer	263	30749.1	1	UniProt (Q0E856) Pfam (PF01051) Pfam (PF21205) UniProt (by SIFTS) (P03856)	Escherichia coli	RepE,RepE protein,Replication initiation protein,Replication initiation protein FIA,Replication initiation protein RepE,Replication initiation protein of repFIA,FIA,FIA replicon,Replication protein
4	D (G)	Unidentified polymer	polymer	3	273.3	1		Eschericia coli
5	E (D)	Unidentified polymer	polymer	4	358.4	1		Eschericia coli
6	F, G, H (C)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
7	I, J, K (A, B, C)	water	water		18.0	27	Chemie (HOH)

Sequence modifications

C: 1 - 251 (UniProt: Q0E856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 118	Arg 118	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	44267.2
Non-Polymers*	Number of molecules	3
	Total formula weight	72.9
All*	Total formula weight	44340.1

*Water molecules are not included.