7SDE

Cryo-EM structure of Nse5/6 heterodimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Non-structural maintenance of chromosome element 5	polymer	555	63980.5	1	UniProt (Q03718) Pfam (PF08691)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Non-SMC element 5
2	B (B)	Ubiquitin-like protein SMT3,DNA repair protein KRE29 chimera	polymer	586	67645.2	1	UniProt (Q12306) UniProt (P40026) Pfam (PF00240) Pfam (PF08691)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	6XHis-SUMO-Nse6

Sequence modifications

A: 1 - 556 (UniProt: Q03718)

PDB	External Database	Details
-	Val 263	deletion

B: -98 - -1 (UniProt: Q12306)

PDB	External Database	Details
Met -121	-	expression tag
Gly -120	-	expression tag
Ser -119	-	expression tag
Ser -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
His -114	-	expression tag
His -113	-	expression tag
His -112	-	expression tag
Ser -111	-	expression tag
Ser -110	-	expression tag
Gly -109	-	expression tag
Leu -108	-	expression tag
Val -107	-	expression tag
Pro -106	-	expression tag
Arg -105	-	expression tag
Gly -104	-	expression tag
Ser -103	-	expression tag
His -102	-	expression tag
Met -101	-	expression tag
Ala -100	-	expression tag
Ser -99	-	expression tag
Ser 0	-	linker

B: 1 - 464 (UniProt: P40026)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	131625.7
All*	Total formula weight	131625.7

*Water molecules are not included.