7SCK

Cryo-EM structure of the human Exostosin-1 and Exostosin-2 heterodimer in complex with a 7-sugar oligosaccharide acceptor analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Exostosin-1	polymer	720	83404.0	1	UniProt (Q16394) Pfam (PF03016) Pfam (PF09258)	Homo sapiens (human)	Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase,Multiple exostoses protein 1,Putative tumor suppressor protein EXT1
2	B (B)	Exostosin-2	polymer	674	77238.0	1	UniProt (Q93063) Pfam (PF03016) Pfam (PF09258)	Homo sapiens (human)	Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase,Multiple exostoses protein 2,Putative tumor suppressor protein EXT2
3	C (C)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	D (D)	beta-D-glucopyranuronic acid-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose	branched		776.6	1	In PDB
5	E, G (A, B)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP)
6	F (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
7	H (B)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)

Sequence modifications

A: 28 - 746 (UniProt: Q16394)

PDB	External Database	Details
Gly 27	-	expression tag

B: 46 - 718 (UniProt: Q93063)

PDB	External Database	Details
Gly 45	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	160642.1
Branched	Number of molecules	2
	Total formula weight	1363.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1084.5
All*	Total formula weight	163089.7

*Water molecules are not included.