7S4X
Cas9:gRNA in complex with 18-20MM DNA, 1 minute time-point, kinked active conformation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158699.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 2 | B (B) | gRNA | polymer | 98 | 31702.9 | 1 | synthetic construct | ||
| 3 | C (C) | TS | polymer | 16 | 4865.2 | 1 | synthetic construct | ||
| 4 | D (c) | TS | polymer | 20 | 6059.9 | 1 | synthetic construct | ||
| 5 | E (D) | NTS | polymer | 36 | 10091.2 | 1 | synthetic construct | ||
| 6 | F, G, H, I, J (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 7 | K, L, M (A, B, c) | water | water | 18.0 | 7 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 211419.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 121.5 | |
| All* | Total formula weight | 211540.6 |
