7S47

Integrin beta5(743-774)-linker-PAK4cat(D440N/S474E)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Integrin beta-5, Isoform 2 of Serine/threonine-protein kinase PAK 4 chimera	polymer	383	43060.6	1	UniProt (P18084) UniProt (O96013-2) Pfam (PF08725) Pfam (PF00069) UniProt (by SIFTS) (O96013) In PDB	Homo sapiens (Human)	p21-activated kinase 4,PAK-4
2	B	Integrin beta-5	polymer	32	3921.5	1	UniProt (P18084) Pfam (PF08725) In PDB	Homo sapiens (Human)
3	A	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
4		water	water		18.0	103	Chemie (HOH)

Sequence modifications

A: 230 - 261 (UniProt: P18084)

PDB	External Database	Details
Met 209	-	initiating methionine
Gly 210	-	expression tag
Ser 211	-	expression tag
Ser 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag
His 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
Ser 219	-	expression tag
Ser 220	-	expression tag
Gly 221	-	expression tag
Leu 222	-	expression tag
Val 223	-	expression tag
Pro 224	-	expression tag
Arg 225	-	expression tag
Gly 226	-	expression tag
Ser 227	-	expression tag
His 228	-	expression tag
Met 229	-	expression tag

A: 262 - 273 (PDB: 7S47)
A: 274 - 591 (UniProt: O96013-2)

PDB	External Database	Details
Asn 440	Asp 275	engineered mutation
Glu 474	Ser 309	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	46982.1
Non-Polymers*	Number of molecules	1
	Total formula weight	506.2
All*	Total formula weight	47488.3

*Water molecules are not included.