7S36
Cas9:sgRNA:DNA (S. pyogenes) with 0 RNA:DNA base pairs, closed-protein/bent-DNA conformation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | N | Non-target DNA strand | polymer | 30 | 9314.0 | 1 | synthetic construct | ||
| 2 | P | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1371 | 158974.1 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB | Streptococcus pyogenes serotype M1 | SpCas9,SpyCas9 |
| 3 | R | Single-guide RNA | polymer | 102 | 32787.4 | 1 | Streptococcus pyogenes | ||
| 4 | T | Target DNA strand | polymer | 30 | 9191.0 | 1 | synthetic construct |
Sequence modifications
P: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Cys 1337 | Thr 1337 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 210266.5 | |
| All* | Total formula weight | 210266.5 |
