7RZR

Cryo-EM structure of the SARS-CoV-2 HR1HR2 fusion core complex with D936Y mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	SARS-CoV-2 HR1 D936Y linked to a scaffold,Spike protein S2'	polymer	257	28807.1	3	UniProt (B2J981) UniProt (P0DTC2) Pfam (PF00210)	Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
2	D, E, F (D, E, F)	Spike protein S2'	polymer	41	4422.9	3	UniProt (P0DTC2)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)

Sequence modifications

A, B, C: 742 - 915 (UniProt: B2J981)

PDB	External Database	Details
Met 732	-	initiating methionine
Ser 733	-	expression tag
His 734	-	expression tag
His 735	-	expression tag
His 736	-	expression tag
His 737	-	expression tag
His 738	-	expression tag
His 739	-	expression tag
Gly 740	-	expression tag
Ser 741	-	expression tag
Ala 916	-	linker

A, B, C: 917 - 988 (UniProt: P0DTC2)

PDB	External Database	Details
Tyr 936	Asp 936	engineered mutation

D, E, F: 1162 - 1201 (UniProt: P0DTC2)

PDB	External Database	Details
Gly 1161	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	99690.1
All*	Total formula weight	99690.1

*Water molecules are not included.