7RY4
Multi-conformer model of Ketosteroid Isomerase Y57F/D40N mutant from Pseudomonas Putida (pKSI) bound to a transition state analog at 250 K
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Steroid Delta-isomerase | polymer | 131 | 14531.5 | 2 | UniProt (P07445) | Pseudomonas putida (Arthrobacter siderocapsulatus) | Delta(5)-3-ketosteroid isomerase |
| 2 | C, H (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) PubChem (312) | |||
| 3 | D, E, F, I, J... (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 4 | G, L (A, B) | (9beta,13alpha)-3-hydroxyestra-1,3,5(10)-trien-17-one | non-polymer | 270.4 | 2 | Chemie (J3Z) PubChem (698) PubChem (5870) PubChem (46781514) PubChem (133184) PubChem (56619853) PubChem (68209946) PubChem (71774672) PubChem (657358) PubChem (667482) PubChem (670995) PubChem (928336) PubChem (7059423) PubChem (5702052) PubChem (6336180) PubChem (6540830) PubChem (6603847) PubChem (6710658) PubChem (6918970) PubChem (6954895) PubChem (7059424) PubChem (9547221) PubChem (10989336) PubChem (11869378) PubChem (11869537) PubChem (12305076) PubChem (133638141) PubChem (141767110) PubChem (143400863) PubChem (145187136) | |||
| 5 | M, N (A, B) | water | water | 18.0 | 249 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 131 (UniProt: P07445)
| PDB | External Database | Details |
|---|---|---|
| Asn 40 | Asp 40 | engineered mutation |
| Phe 57 | Tyr 57 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 29063.0 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 757.5 | |
| All* | Total formula weight | 29820.5 |
