7RY4
Multi-conformer model of Ketosteroid Isomerase Y57F/D40N mutant from Pseudomonas Putida (pKSI) bound to a transition state analog at 250 K
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Steroid Delta-isomerase | polymer | 131 | 14531.5 | 2 | UniProt (P07445) Pfam (PF12680) | Pseudomonas putida (Arthrobacter siderocapsulatus) | Delta(5)-3-ketosteroid isomerase |
| 2 | C, H (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 3 | D, E, F, I, J... (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
| 4 | G, L (A, B) | (9beta,13alpha)-3-hydroxyestra-1,3,5(10)-trien-17-one | non-polymer | 270.4 | 2 | Chemie (J3Z) | |||
| 5 | M, N (A, B) | water | water | 18.0 | 249 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 131 (UniProt: P07445)
| PDB | External Database | Details |
|---|---|---|
| Asn 40 | Asp 40 | engineered mutation |
| Phe 57 | Tyr 57 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 29063.0 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 757.5 | |
| All* | Total formula weight | 29820.5 |
