7RSM
Crystal structure of pyrrolysyl-tRNA synthetase (N346D/C348S/Y384F) in complex with o-Chlorophenylalanine and AMP-PNP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Pyrrolysine--tRNA ligase | polymer | 267 | 30853.4 | 4 | UniProt (A0A0F8JXW8) Pfam (PF01409) UniProt (by SIFTS) (Q8PWY1) | Methanosarcina mazei (Methanosarcina frisia) | Pyrrolysyl-tRNA synthetase,PylRS |
| 2 | E, G, I, K (A, B, C, D) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 4 | Chemie (ANP) | |||
| 3 | F, H, J, L (A, B, C, D) | 2-chloro-L-phenylalanine | non-polymer | 199.6 | 4 | Chemie (2L5) | |||
| 4 | M, N, O, P (A, B, C, D) | water | water | 18.0 | 333 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 188 - 454 (UniProt: A0A0F8JXW8)
| PDB | External Database | Details |
|---|---|---|
| Asp 346 | Asn 346 | engineered mutation |
| Ser 348 | Cys 348 | engineered mutation |
| Phe 384 | Tyr 384 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 123413.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 2823.3 | |
| All* | Total formula weight | 126236.8 |
