7RRI

Crystal structure of fast switching S142A/M159T mutant of fluorescent protein Dronpa (Dronpa2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Fluorescent protein Dronpa	polymer	257	28945.6	8	UniProt (Q5TLG6) Pfam (PF01353) In PDB	Echinophyllia sp. SC22
2		water	water		18.0	257	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 3 - 229 (UniProt: Q5TLG6)

PDB	External Database	Details
Gly -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
Gly -16	-	expression tag
Leu -15	-	expression tag
Val -14	-	expression tag
Pro -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
Met -8	-	expression tag
Val -7	-	expression tag
Ser -6	-	expression tag
Lys -5	-	expression tag
Gly -4	-	expression tag
Glu -3	-	expression tag
Glu -2	-	expression tag
Asn -1	-	expression tag
Asn 0	-	expression tag
Met 1	-	expression tag
Ala 2	-	expression tag
Gyc 63	Cys 62	chromophore
Gyc 63	Tyr 63	chromophore
Gyc 63	Gly 64	chromophore
Ala 142	Ser 142	engineered mutation
Thr 159	Met 159	engineered mutation
Gly 218	Glu 218	conflict
Met 224	-	insertion
Asp 225	-	insertion
Glu 226	-	insertion
Leu 227	-	insertion
Tyr 228	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	231564.6
All*	Total formula weight	231564.6

*Water molecules are not included.