7ROF

Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB2B9-H275E with L-Trp non-covalently bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Tryptophan synthase beta chain 1	polymer	396	43609.7	4	UniProt (Q8U093) Pfam (PF00291)	Pyrococcus furiosus
2	E, I (A, D)	TRYPTOPHAN	non-polymer		204.2	2	Chemie (TRP)
3	F, G, H, J (A, B, C, D)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
4	K, L, M, N (A, B, C, D)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 388 (UniProt: Q8U093)

PDB	External Database	Details
Val 16	Ile 16	conflict
Gly 17	Glu 17	conflict
Val 68	Ile 68	conflict
Leu 95	Phe 95	conflict
Ser 274	Phe 274	conflict
Glu 275	His 275	engineered mutation
Ser 292	Thr 292	conflict
Ala 321	Thr 321	conflict
Ala 384	Val 384	conflict
Leu 389	-	expression tag
Glu 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	174438.8
Non-Polymers*	Number of molecules	6
	Total formula weight	500.4
All*	Total formula weight	174939.2

*Water molecules are not included.