7RMH
Substance P bound to active human neurokinin 1 receptor in complex with miniGs399
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 248 | 28907.7 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (Human) | miniGs399, Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 370 | 40786.6 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (Human) | Transducin beta chain 1 |
3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7563.8 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
4 | N | Nanobody 35 | polymer | 142 | 15398.1 | 1 | Lama glama | ||
5 | R | Substance-P receptor | polymer | 418 | 47542.9 | 1 | UniProt (P25103) Pfam (PF00001) In PDB | Homo sapiens (Human) | SPR,NK-1 receptor,NK-1R,Tachykinin receptor 1 |
6 | S | Substance P | polymer | 12 | 1348.6 | 1 | UniProt (P20366) Pfam (PF02202) In PDB | Homo sapiens |
Sequence modifications
A: 6 - 195 (UniProt: P63092)
A: 204 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
R: 1 - 407 (UniProt: P25103)
S: 1 - 11 (UniProt: P20366)
PDB | External Database | Details |
---|---|---|
Asp 49 | Gly 49 | engineered mutation |
Asn 50 | Glu 50 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 196 | - | linker |
Gly 197 | - | linker |
Ser 198 | - | linker |
Gly 199 | - | linker |
Gly 200 | - | linker |
Ser 201 | - | linker |
Gly 202 | - | linker |
Gly 203 | - | linker |
Asp 249 | Ala 249 | engineered mutation |
Asp 252 | Ser 252 | engineered mutation |
- | Asn 254 | deletion |
- | Met 255 | deletion |
- | Val 256 | deletion |
- | Ile 257 | deletion |
- | Arg 258 | deletion |
- | Glu 259 | deletion |
- | Asp 260 | deletion |
- | Asn 261 | deletion |
- | Gln 262 | deletion |
- | Thr 263 | deletion |
Ala 372 | Ile 372 | engineered mutation |
Ile 375 | Val 375 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -29 | - | initiating methionine |
His -28 | - | expression tag |
His -27 | - | expression tag |
His -26 | - | expression tag |
His -25 | - | expression tag |
His -24 | - | expression tag |
His -23 | - | expression tag |
Leu -22 | - | expression tag |
Glu -21 | - | expression tag |
Val -20 | - | expression tag |
Leu -19 | - | expression tag |
Phe -18 | - | expression tag |
Gln -17 | - | expression tag |
Gly -16 | - | expression tag |
Pro -15 | - | expression tag |
Glu -14 | - | expression tag |
Asp -13 | - | expression tag |
Gln -12 | - | expression tag |
Val -11 | - | expression tag |
Asp -10 | - | expression tag |
Pro -9 | - | expression tag |
Arg -8 | - | expression tag |
Leu -7 | - | expression tag |
Ile -6 | - | expression tag |
Asp -5 | - | expression tag |
Gly -4 | - | expression tag |
Lys -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Asp -10 | - | expression tag |
Tyr -9 | - | expression tag |
Lys -8 | - | expression tag |
Asp -7 | - | expression tag |
Asp -6 | - | expression tag |
Asp -5 | - | expression tag |
Asp -4 | - | expression tag |
Ala -3 | - | expression tag |
Ser -2 | - | expression tag |
Ile -1 | - | expression tag |
Asp 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Nh2 12 | - | amidation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 141547.6 | |
All* | Total formula weight | 141547.6 |