7RKX

Structure of US27-Gi-scFv16 in CL-state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	353	40283.8	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37728.2	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	67	7432.6	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (D)	Antibody fragment scFv16	polymer	256	27340.5	1		Mus musculus (mouse)
5	E (R)	G-protein coupled receptor homolog US27	polymer	382	44451.7	1	UniProt (P09703) Pfam (PF00001)	Human cytomegalovirus (HHV-5 Human herpesvirus 5 Human betaherpesvirus 5)	HHRF2

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 1 - 362 (UniProt: P09703)

PDB	External Database	Details
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Ser 363	-	expression tag
Arg 364	-	expression tag
Gly 365	-	expression tag
Ala 366	-	expression tag
Ala 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	157236.8
All*	Total formula weight	157236.8

*Water molecules are not included.