7RGP

cryo-EM of human Glucagon-like peptide 1 receptor GLP-1R bound to tirzepatide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-3,Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	373	43385.2	1	UniProt (P08754) UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	G(i) alpha-3,Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (E)	Single-chain variable fragment 16	polymer	297	31870.6	1		Mus musculus
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (N)	nanobody 35	polymer	160	17352.5	1		Lama glama
6	F (P)	Tirzepatide	polymer	39	4072.5	1		Homo sapiens
7	G (R)	Glucagon-like peptide 1 receptor	polymer	445	51233.1	1	UniProt (P43220) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (human)	GLP-1R

Sequence modifications

A: 8 - 25 (UniProt: P08754)
A: 26 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 24 - 422 (UniProt: P43220)

PDB	External Database	Details
Met -22	-	initiating methionine
Lys -21	-	expression tag
Thr -20	-	expression tag
Ile -19	-	expression tag
Ile -18	-	expression tag
Ala -17	-	expression tag
Leu -16	-	expression tag
Ser -15	-	expression tag
Tyr -14	-	expression tag
Ile -13	-	expression tag
Phe -12	-	expression tag
Cys -11	-	expression tag
Leu -10	-	expression tag
Val -9	-	expression tag
Phe -8	-	expression tag
Ala -7	-	expression tag
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Ala 1	-	expression tag
Ala 2	-	expression tag
Ala 3	-	expression tag
Gly 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag
Gly 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Leu 10	-	expression tag
Glu 11	-	expression tag
Val 12	-	expression tag
Leu 13	-	expression tag
Phe 14	-	expression tag
Gln 15	-	expression tag
Gly 16	-	expression tag
Pro 17	-	expression tag
Gly 18	-	expression tag
Gly 19	-	expression tag
Ser 20	-	expression tag
Gly 21	-	expression tag
Gly 22	-	expression tag
Ser 23	-	expression tag
Phe 260	Leu 260	variant

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	194309.2
All*	Total formula weight	194309.2

*Water molecules are not included.