7RBT

cryo-EM structure of human Gastric inhibitory polypeptide receptor GIPR bound to tirzepatide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	373	43385.2	1	UniProt (P63092-2) Pfam (PF00503) UniProt (by SIFTS) (P63096) UniProt (by SIFTS) (P63092) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	E	Single-chain variable fragment 16	polymer	297	31870.6	1		Mus musculus
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
5	N	nanobody 35	polymer	160	17352.5	1		Lama glama
6	P	Tirzepatide	polymer	39	4072.5	1		Homo sapiens
7	R	Gastric inhibitory polypeptide receptor	polymer	463	52871.8	1	UniProt (P48546) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (Human)	GIP-R,Glucose-dependent insulinotropic polypeptide receptor
8	R	2-fluoro-4-[(1R)-6-methoxy-1-methyl-2-{(1S)-1-[4-(propan-2-yl)phenyl]ethyl}-1,2,3,4-tetrahydroisoquinolin-5-yl]-6-[(2-methylpropyl)amino]phenol	non-polymer		504.7	1	Chemie (41Y)

Sequence modifications

A: 26 - 394 (UniProt: P63092-2)

PDB	External Database	Details
Met 8	-	initiating methionine
Gly 9	-	expression tag
Cys 10	-	expression tag
Thr 11	-	expression tag
Leu 12	-	expression tag
Ser 13	-	expression tag
Ala 14	-	expression tag
Glu 15	-	expression tag
Asp 16	-	expression tag
Lys 17	-	expression tag
Ala 18	-	expression tag
Ala 19	-	expression tag
Val 20	-	expression tag
Glu 21	-	expression tag
Arg 22	-	expression tag
Ser 23	-	expression tag
Lys 24	-	expression tag
Met 25	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 22 - 466 (UniProt: P48546)

PDB	External Database	Details
Asp 4	-	expression tag
Tyr 5	-	expression tag
Lys 6	-	expression tag
Asp 7	-	expression tag
Asp 8	-	expression tag
Asp 9	-	expression tag
Asp 10	-	expression tag
Ala 11	-	expression tag
Ala 12	-	expression tag
Ala 13	-	expression tag
Leu 14	-	expression tag
Glu 15	-	expression tag
Val 16	-	expression tag
Leu 17	-	expression tag
Phe 18	-	expression tag
Gln 19	-	expression tag
Gly 20	-	expression tag
Pro 21	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	195947.9
Non-Polymers*	Number of molecules	1
	Total formula weight	504.7
All*	Total formula weight	196452.6

*Water molecules are not included.