7RAI
Cryo-EM structure of M4008_N1 Fab in complex with BG505 DS-SOSIP.664 Env trimer
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I (A, C, E) | Envelope glycoprotein gp160 | polymer | 476 | 53300.3 | 3 | UniProt (Q2N0S6) Pfam (PF00516) | Human immunodeficiency virus 1 | Env polyprotein |
| 2 | B, F, J (B, D, F) | HIV-1 Envelope Glycoprotein BG505 SOSIP.664 gp41 | polymer | 153 | 17146.5 | 3 | UniProt (Q2N0S6) Pfam (PF00517) | Human immunodeficiency virus 1 (HIV-1) | |
| 3 | C, G, K (H, I, J) | M4008_N1 Fab heavy chain | polymer | 264 | 28016.7 | 3 | Homo sapiens | ||
| 4 | D, H, L (L, M, N) | M4008_N1 Fab light chain | polymer | 216 | 23628.4 | 3 | Homo sapiens | ||
| 5 | KA, M, Y (k, G, Y) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 3 | In PDB GlyTouCan (G22768VO) | |||
| 6 | GA, HA, IA, JA, LA... (g, h, i, j, l...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 15 | In PDB GlyTouCan (G42666HT) | |||
| 7 | AA, CA, DA, EA, FA... (a, c, d, e, f...) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 15 | In PDB GlyTouCan (G15407YE) | |||
| 8 | BA, NA, P (b, n, P) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 3 | In PDB GlyTouCan (G46836GH) | |||
| 9 | AB, BB, CB, DB, EB... (A, L, C, M, E...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 21 | Chemie (NAG) |
Sequence modifications
A, C, E: 31 - 508 (UniProt: Q2N0S6)
B, D, F: 512 - 664 (UniProt: Q2N0S6)
| PDB | External Database | Details |
|---|---|---|
| Cys 201 | Ile 200 | engineered mutation |
| Asn 332 | Thr 330 | engineered mutation |
| Cys 433 | Ala 430 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | engineered mutation |
| Cys 605 | Thr 602 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 366275.7 | |
| Branched | Number of molecules | 36 |
| Total formula weight | 21601.9 | |
| Non-Polymers* | Number of molecules | 21 |
| Total formula weight | 4645.4 | |
| All* | Total formula weight | 392523.0 |
