7RAG

Structure of the CwlD amidase from Clostridioides difficile in complex with the GerS lipoprotein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Lipoprotein	polymer	175	20823.2	1	UniProt (A0A031WJD5) UniProt (by SIFTS) (Q180W3) In PDB	Clostridioides difficile (Peptoclostridium difficile)	Lipoprotein localisation LolA/LolB/LppX,Outer membrane lipoprotein-sorting protein,Putative lipoprotein
2	B	Germination-specific N-acetylmuramoyl-L-alanine amidase, Autolysin	polymer	217	24951.9	1	UniProt (Q18CJ4) Pfam (PF01520) In PDB	Clostridioides difficile (Peptoclostridium difficile)
3	B	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5		water	water		18.0	14	Chemie (HOH)

Sequence modifications

A: 23 - 196 (UniProt: A0A031WJD5)

PDB	External Database	Details
Mse 22	-	initiating methionine

B: 26 - 234 (UniProt: Q18CJ4)

PDB	External Database	Details
Mse 25	-	initiating methionine
Val 235	-	expression tag
Asp 236	-	expression tag
Lys 237	-	expression tag
Leu 238	-	expression tag
Ala 239	-	expression tag
Ala 240	-	expression tag
Ala 241	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45775.1
Non-Polymers*	Number of molecules	2
	Total formula weight	127.5
All*	Total formula weight	45902.5

*Water molecules are not included.