7R5Q

Escherichia coli type II Asparaginase N24S mutant in complex with GLU

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	L-asparaginase 2	polymer	332	35428.7	4	UniProt (P00805) Pfam (PF00710) Pfam (PF17763) In PDB	Escherichia coli K-12	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	A, B, C, D	GLUTAMIC ACID	non-polymer		147.1	4	Chemie (GLU)
3		water	water		18.0	519	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ser 24	Asn 46	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	141714.6
Non-Polymers*	Number of molecules	4
	Total formula weight	588.5
All*	Total formula weight	142303.2

*Water molecules are not included.