7R2K
elongated Cascade complex from type I-A CRISPR-Cas system
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated helicase Cas3 | polymer | 651 | 74475.7 | 1 | UniProt (A0A5C0XNV5) Pfam (PF00270) Pfam (PF22590) | Pyrococcus furiosus DSM 3638 | |
| 2 | B, D, E, F, G... (C, E, F, G, H...) | Cas11a | polymer | 108 | 12208.9 | 7 | UniProt (Q8U332) | Pyrococcus furiosus DSM 3638 | |
| 3 | C (D) | DNA (5'-D(P*TP*AP*CP*AP*AP*GP*GP*GP*A)-3') | polymer | 9 | 2788.9 | 1 | Escherichia coli | ||
| 4 | J, K, L, M, N... (K, L, M, N, O...) | Cas7a | polymer | 336 | 36989.1 | 10 | UniProt (Q8U333) Pfam (PF01905) | Pyrococcus furiosus DSM 3638 | |
| 5 | P (Q) | CRISPR-associated endonuclease Cas3-HD | polymer | 238 | 27130.0 | 1 | UniProt (Q8U336) Pfam (PF18019) | Pyrococcus furiosus DSM 3638 | CRISPR-associated ss-nucleic acid exo- and endonuclease Cas3-HD |
| 6 | R (S) | Type I-A CRISPR-associated protein Cas5 | polymer | 256 | 29147.2 | 1 | UniProt (A0A5C0XNV9) | Pyrococcus furiosus DSM 3638 | |
| 7 | T (U) | crRNA (57-MER) | polymer | 57 | 18389.9 | 1 | Escherichia coli | ||
| 8 | W (X) | DNA (5'-D(P*TP*CP*CP*C)-3') | polymer | 4 | 1126.8 | 1 | Escherichia coli | ||
| 9 | X (Y) | Cas8 | polymer | 341 | 38800.7 | 1 | UniProt (Q8U338) Pfam (PF09703) | Pyrococcus furiosus DSM 3638 | |
| 10 | Y, Z (Q) | NICKEL (II) ION | non-polymer | 58.7 | 2 | Chemie (NI) |
Sequence modifications
A: 10 - 659 (UniProt: A0A5C0XNV5)
Q: 2 - 236 (UniProt: Q8U336)
S: 1 - 256 (UniProt: A0A5C0XNV9)
Y: 2 - 342 (UniProt: Q8U338)
| PDB | External Database | Details |
|---|---|---|
| Lys 229 | Arg 228 | conflict |
| Val 393 | Thr 393 | conflict |
| Pro 451 | - | insertion |
| Lys 517 | Leu 517 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 237 | - | expression tag |
| Ala 238 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| - | Thr 235 | deletion |
| PDB | External Database | Details |
|---|---|---|
| Val 24 | Glu 25 | conflict |
| Ser 64 | Glu 65 | conflict |
| Leu 110 | Val 111 | conflict |
| - | Ser 235 | deletion |
| - | Leu 236 | deletion |
| - | Gly 237 | deletion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 24 |
| Total formula weight | 647213.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 117.4 | |
| All* | Total formula weight | 647330.6 |
