7QV9
CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (N) | Non-template promoter DNA | polymer | 63 | 19439.4 | 1 | Klebsiella pneumoniae | ||
| 6 | G (T) | Template promoter DNA | polymer | 63 | 19404.4 | 1 | Klebsiella pneumoniae | ||
| 7 | H, I, J, K, L... (a, b, c, d, f...) | Transcription activator PspF | polymer | 295 | 33187.8 | 6 | Pfam (PF00158) UniProt (by SIFTS) (P37344) | Escherichia coli K-12 | |
| 8 | N (M) | RNA polymerase sigma-54 factor | polymer | 477 | 53978.7 | 1 | UniProt (A0A0N9UTC1) Pfam (PF00309) Pfam (PF04963) Pfam (PF04552) UniProt (by SIFTS) (A0A0H3H3L1) | Klebsiella pneumoniae | |
| 9 | O, Q, S, T, V (a, b, c, f, e) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 5 | Chemie (ADP) | |||
| 10 | P, R, U, W (a, b, f, e) | ALUMINUM FLUORIDE | non-polymer | 84.0 | 4 | Chemie (AF3) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 681503.7 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2471.9 | |
| All* | Total formula weight | 683975.6 |
