7QTM
Transition state analogue of small G protein in complex with relevant GAP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, H) | Rho GTPase-activating protein 1 | polymer | 244 | 27502.5 | 2 | UniProt (Q07960) Pfam (PF00620) | Homo sapiens (human) | CDC42 GTPase-activating protein,GTPase-activating protein rhoGAP,Rho-related small GTPase protein activator,Rho-type GTPase-activating protein 1,p50-RhoGAP |
| 2 | B, D (B, I) | Transforming protein RhoA | polymer | 192 | 21670.9 | 2 | UniProt (P61586) Pfam (PF00071) | Homo sapiens (human) | Rho cDNA clone 12,h12 |
| 3 | E, H (B, I) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 4 | F, I (B, I) | TRIFLUOROMAGNESATE | non-polymer | 81.3 | 2 | Chemie (MGF) | |||
| 5 | G, J (B, I) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 6 | K, L, M, N (A, B, H, I) | water | water | 18.0 | 165 | Chemie (HOH) |
Sequence modifications
A, H: 1 - 242 (UniProt: Q07960)
B, I: 2 - 193 (UniProt: P61586)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 85 | Arg 282 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asn 25 | Phe 25 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 98346.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1097.6 | |
| All* | Total formula weight | 99444.3 |
