7QR8
SpCas9 bound to PTPRC off-target1 DNA substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | PTPRC sgRNA | polymer | 85 | 27363.4 | 1 | synthetic construct | ||
| 2 | B | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158588.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 3 | C | PTPRC off-target1 target strand | polymer | 28 | 8707.6 | 1 | synthetic construct | ||
| 4 | D | PTPRC off-target1 non-target strand | polymer | 12 | 3629.4 | 1 | synthetic construct | ||
| 5 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | A, B, C | POTASSIUM ION | non-polymer | 39.1 | 10 | Chemie (K) | |||
| 7 | water | water | 18.0 | 191 | Chemie (HOH) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 198289.1 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 439.6 | |
| All* | Total formula weight | 198728.7 |
