7QR1
SpCas9 bound to TRAC off-target2 DNA substrate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | TRAC sgRNA | polymer | 84 | 27133.2 | 1 | synthetic construct | ||
2 | B | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158588.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB | Streptococcus pyogenes | SpCas9,SpyCas9 |
3 | C | TRAC off-target2 target strand | polymer | 28 | 8555.5 | 1 | synthetic construct | ||
4 | D | TRAC off-target2 non-target strand | polymer | 12 | 3692.4 | 1 | synthetic construct | ||
5 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
6 | A, B | POTASSIUM ION | non-polymer | 39.1 | 14 | Chemie (K) | |||
7 | water | water | 18.0 | 301 | Chemie (HOH) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
PDB | External Database | Details |
---|---|---|
Ala 10 | Asp 10 | engineered mutation |
Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 197969.9 | |
Non-Polymers* | Number of molecules | 16 |
Total formula weight | 596.0 | |
All* | Total formula weight | 198565.9 |