7QOY

A mutant of the nitrile hydratase from Geobacillus pallidus having enhanced thermostability

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Nitrile hydratase	polymer	216	24802.4	1	UniProt (Q84FS5) Pfam (PF02979) In PDB	Aeribacillus pallidus
2	B	Nitrile hydratase subunit beta	polymer	229	26532.1	1	UniProt (Q84FS6) Pfam (PF21006) Pfam (PF02211) In PDB	Aeribacillus pallidus	NHase
3	A	COBALT (III) ION	non-polymer		58.9	1	Chemie (3CO)
4	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5		water	water		18.0	427	Chemie (HOH)

Sequence modifications

A: 1 - 216 (UniProt: Q84FS5)

PDB	External Database	Details
Arg 169	Ser 169	engineered mutation

B: 1 - 229 (UniProt: Q84FS6)

PDB	External Database	Details
Lys 43	Met 43	engineered mutation
Ala 150	Thr 150	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51334.5
Non-Polymers*	Number of molecules	2
	Total formula weight	94.4
All*	Total formula weight	51428.8

*Water molecules are not included.