7QOU

A mutant of the nitrile hydratase from Geobacillus pallidus having enhanced thermostability

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Nitrile hydratase	polymer	216	24700.2	1	UniProt (Q84FS5) Pfam (PF02979) In PDB	Aeribacillus pallidus
2	B	Nitrile hydratase subunit beta	polymer	229	26562.2	1	UniProt (Q84FS6) Pfam (PF21006) Pfam (PF02211) In PDB	Aeribacillus pallidus	NHase
3	A	COBALT (II) ION	non-polymer		58.9	1	Chemie (CO)
4	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6		water	water		18.0	391	Chemie (HOH)

Sequence modifications

A: 1 - 216 (UniProt: Q84FS5)

PDB	External Database	Details
Val 188	Met 188	engineered mutation

B: 1 - 229 (UniProt: Q84FS6)

PDB	External Database	Details
Glu 96	Asp 96	engineered mutation
Val 167	Asp 167	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51262.4
Non-Polymers*	Number of molecules	4
	Total formula weight	143.0
All*	Total formula weight	51405.4

*Water molecules are not included.