7QNF

CRYSTAL STRUCTURE OF E.coli ALCOHOL DEHYDROGENASE - FucO MUTANT N151G, L259V COMPLEXED WITH FE, NAD+, AND ETHYLENE GLYCOL

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (AAA, BBB)	Lactaldehyde reductase	polymer	390	41464.2	2	UniProt (P0A9S2) Pfam (PF00465) Pfam (PF25137) UniProt (by SIFTS) (P0A9S1)	Escherichia coli str. K-12 substr. MG1655	Propanediol oxidoreductase
2	C, K (AAA, BBB)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
3	D, E, F, H, L... (AAA, BBB)	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
4	G, J (AAA)	FORMIC ACID	non-polymer		46.0	2	Chemie (FMT)
5	I, O (AAA, BBB)	ADENOSINE-5-DIPHOSPHORIBOSE	non-polymer		559.3	2	Chemie (APR)
6	P, Q (AAA, BBB)	water	water		18.0	266	Chemie (HOH)

Sequence modifications

AAA, BBB: 2 - 383 (UniProt: P0A9S2)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 151	Asn 150	engineered mutation
Val 259	Leu 258	engineered mutation
Gly 315	Ser 314	engineered mutation
Thr 384	-	expression tag
Ser 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag
His 389	-	expression tag
His 390	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	82928.5
Non-Polymers*	Number of molecules	13
	Total formula weight	1756.8
All*	Total formula weight	84685.3

*Water molecules are not included.