7QN9
Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with GABA, histamine and nanobody Nb25 in a pre-open/closed state
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Gamma-aminobutyric acid receptor subunit alpha-4 | polymer | 554 | 61696.0 | 1 | UniProt (P48169) Pfam (PF02931) Pfam (PF02932) In PDB | Homo sapiens (human) | GABA(A) receptor subunit alpha-4 |
2 | B, C, D | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 473 | 54180.3 | 3 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) In PDB | Homo sapiens (human) | GABA(A) receptor subunit beta-3 |
3 | E | Gamma-aminobutyric acid receptor subunit delta | polymer | 472 | 52437.9 | 1 | UniProt (O14764) Pfam (PF02931) Pfam (PF02932) In PDB | Homo sapiens (human) | GABA(A) receptor subunit delta |
4 | L, M | Nanobody Nb25 | polymer | 121 | 13341.7 | 2 | Homo sapiens | ||
5 | a | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
6 | b, c, d | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
7 | F, G, H | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 3 | In PDB GlyTouCan (G22768VO) | |||
8 | A, E | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
9 | B, C, D | HISTAMINE | non-polymer | 111.1 | 3 | Chemie (HSM) | |||
10 | B | HEXADECANE | non-polymer | 226.4 | 1 | Chemie (R16) | |||
11 | D | DECANE | non-polymer | 142.3 | 1 | Chemie (D10) |
Sequence modifications
E: 1 - 452 (UniProt: O14764)
PDB | External Database | Details |
---|---|---|
Gly 453 | - | expression tag |
Gly 454 | - | expression tag |
Ser 455 | - | expression tag |
Gly 456 | - | expression tag |
Gly 457 | - | expression tag |
Ser 458 | - | expression tag |
Gly 459 | - | expression tag |
Gly 460 | - | expression tag |
Ser 461 | - | expression tag |
Gly 462 | - | expression tag |
Lys 463 | - | expression tag |
Thr 464 | - | expression tag |
Glu 465 | - | expression tag |
Thr 466 | - | expression tag |
Ser 467 | - | expression tag |
Gln 468 | - | expression tag |
Val 469 | - | expression tag |
Ala 470 | - | expression tag |
Pro 471 | - | expression tag |
Ala 472 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 7 |
Total formula weight | 303358.5 | |
Branched | Number of molecules | 7 |
Total formula weight | 4592.2 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 1144.6 | |
All* | Total formula weight | 309095.3 |