Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7QG8

Structure of the collided E. coli disome - VemP-stalled 70S ribosome

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	A-site tRNA	polymer	73	23546.0	1		Escherichia coli K-12
2	B (s)	VemP nascent chain	polymer	179	20531.1	1		Vibrio alginolyticus
3	C (M)	P-site tRNA	polymer	75	24060.3	1	GenBank (1847035720)	Escherichia coli K-12
4	D (O)	5S rRNA	polymer	120	38790.1	1	GenBank (1845258627)	Escherichia coli K-12
5	E (P)	50S ribosomal protein L2	polymer	273	29923.6	1	UniProt (P60422) Pfam (PF00181) Pfam (PF03947)	Escherichia coli K-12	Large ribosomal subunit protein uL2
6	F (Q)	50S ribosomal protein L3	polymer	209	22277.5	1	UniProt (P60438) Pfam (PF00297)	Escherichia coli K-12	Large ribosomal subunit protein uL3
7	G (R)	50S ribosomal protein L4	polymer	201	22121.6	1	UniProt (P60723) Pfam (PF00573)	Escherichia coli K-12	Large ribosomal subunit protein uL4
8	H (S)	50S ribosomal protein L5	polymer	179	20333.6	1	UniProt (P62399) Pfam (PF00281) Pfam (PF00673)	Escherichia coli K-12	Large ribosomal subunit protein uL5
9	I (T)	50S ribosomal protein L6	polymer	177	18932.8	1	UniProt (P0AG55) Pfam (PF00347)	Escherichia coli K-12	Large ribosomal subunit protein uL6
10	J (U)	50S ribosomal protein L9	polymer	149	15789.0	1	UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948)	Escherichia coli K-12	Large ribosomal subunit protein bL9
11	K (V)	50S ribosomal protein L11	polymer	142	14894.4	1	UniProt (P0A7J7) Pfam (PF03946) Pfam (PF00298)	Escherichia coli K-12	Large ribosomal subunit protein uL11
12	L (W)	50S ribosomal protein L13	polymer	142	16050.6	1	UniProt (P0AA10) Pfam (PF00572)	Escherichia coli K-12	Large ribosomal subunit protein uL13
13	M (X)	50S ribosomal protein L14	polymer	123	13565.1	1	UniProt (P0ADY3) Pfam (PF00238)	Escherichia coli K-12	Large ribosomal subunit protein uL14
14	N (Y)	50S ribosomal protein L15	polymer	144	15008.5	1	UniProt (P02413) Pfam (PF00828)	Escherichia coli K-12	Large ribosomal subunit protein uL15
15	O (Z)	50S ribosomal protein L16	polymer	136	15312.3	1	UniProt (P0ADY7) Pfam (PF00252)	Escherichia coli K-12	Large ribosomal subunit protein uL16
16	P (a)	50S ribosomal protein L17	polymer	127	14335.6	1	UniProt (P0AG44) Pfam (PF01196)	Escherichia coli K-12	Large ribosomal subunit protein bL17
17	Q (b)	50S ribosomal protein L18	polymer	117	12794.7	1	UniProt (P0C018) Pfam (PF00861)	Escherichia coli K-12	Large ribosomal subunit protein uL18
18	R (c)	50S ribosomal protein L19	polymer	115	13159.3	1	UniProt (P0A7K6) Pfam (PF01245)	Escherichia coli K-12	Large ribosomal subunit protein bL19
19	S (d)	50S ribosomal protein L20	polymer	118	13528.0	1	UniProt (P0A7L3) Pfam (PF00453)	Escherichia coli K-12	Large ribosomal subunit protein bL20
20	T (e)	50S ribosomal protein L21	polymer	103	11586.4	1	UniProt (P0AG48) Pfam (PF00829)	Escherichia coli K-12	Large ribosomal subunit protein bL21
21	U (f)	50S ribosomal protein L22	polymer	110	12253.4	1	UniProt (P61175) Pfam (PF00237)	Escherichia coli K-12	Large ribosomal subunit protein uL22
22	V (g)	50S ribosomal protein L23	polymer	100	11252.2	1	UniProt (P0ADZ0) Pfam (PF00276)	Escherichia coli K-12	Large ribosomal subunit protein uL23
23	W (h)	50S ribosomal protein L24	polymer	104	11339.3	1	UniProt (P60624) Pfam (PF00467) Pfam (PF17136)	Escherichia coli K-12	Large ribosomal subunit protein uL24
24	X (i)	50S ribosomal protein L25	polymer	94	10713.5	1	UniProt (P68919) Pfam (PF01386)	Escherichia coli K-12	Large ribosomal subunit protein bL25
25	Y (j)	50S ribosomal protein L27	polymer	85	9093.5	1	UniProt (P0A7L8) Pfam (PF01016)	Escherichia coli K-12	Large ribosomal subunit protein bL27
26	Z (k)	50S ribosomal protein L28	polymer	78	9027.6	1	UniProt (P0A7M2) Pfam (PF00830)	Escherichia coli K-12	Large ribosomal subunit protein bL28
27	AA (l)	50S ribosomal protein L29	polymer	63	7286.5	1	UniProt (P0A7M6) Pfam (PF00831)	Escherichia coli K-12	Large ribosomal subunit protein uL29
28	BA (m)	50S ribosomal protein L30	polymer	59	6554.8	1	UniProt (P0AG51) Pfam (PF00327)	Escherichia coli K-12	Large ribosomal subunit protein uL30
29	CA (n)	50S ribosomal protein L32	polymer	57	6463.4	1	UniProt (P0A7N4) Pfam (PF01783)	Escherichia coli K-12	Large ribosomal subunit protein bL32
30	DA (o)	50S ribosomal protein L33	polymer	55	6388.6	1	UniProt (P0A7N9) Pfam (PF00471)	Escherichia coli K-12	Large ribosomal subunit protein bL33
31	EA (p)	50S ribosomal protein L34	polymer	46	5397.5	1	UniProt (P0A7P5) Pfam (PF00468)	Escherichia coli K-12	Large ribosomal subunit protein bL34
32	FA (q)	50S ribosomal protein L35	polymer	65	7313.0	1	UniProt (P0A7Q1) Pfam (PF01632)	Escherichia coli K-12	Large ribosomal subunit protein bL35,Ribosomal protein A
33	GA (r)	50S ribosomal protein L36	polymer	55	6315.6	1		Escherichia coli K-12
34	HA (N)	23S rRNA	polymer	2903	941306.2	1	GenBank (939732440)	Escherichia coli K-12
35	IA (L)	50S ribosomal protein L31	polymer	70	7917.1	1	UniProt (P0A7M9) Pfam (PF01197)	Escherichia coli K-12	Large ribosomal subunit protein bL31-A
36	JA (C)	50S ribosomal protein L1	polymer	223	23735.5	1	UniProt (P0A7L0) Pfam (PF00687)	Escherichia coli K-12	Large ribosomal subunit protein uL1
37	KA (0)	16S rRNA	polymer	1539	498725.4	1	GenBank (1789840096)	Escherichia coli K-12
38	LA (1)	30S ribosomal protein S2	polymer	239	26581.5	1	UniProt (P0A7V0) Pfam (PF00318)	Escherichia coli K-12	Small ribosomal subunit protein uS2
39	MA (2)	30S ribosomal protein S3	polymer	218	24293.2	1	UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189)	Escherichia coli K-12	Small ribosomal subunit protein uS3
40	NA (3)	30S ribosomal protein S4	polymer	206	23514.2	1	UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479)	Escherichia coli K-12	Small ribosomal subunit protein uS4
41	OA (4)	30S ribosomal protein S5	polymer	162	17120.8	1	UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719)	Escherichia coli K-12	Small ribosomal subunit protein uS5
42	PA (5)	30S ribosomal protein S6, non-modified isoform	polymer	131	15201.0	1	UniProt (P02358) Pfam (PF01250)	Escherichia coli K-12
43	QA (6)	30S ribosomal protein S7	polymer	156	17618.4	1	UniProt (P02359) Pfam (PF00177)	Escherichia coli K-12	Small ribosomal subunit protein uS7
44	RA (7)	30S ribosomal protein S8	polymer	130	14146.6	1	UniProt (P0A7W7) Pfam (PF00410)	Escherichia coli K-12	Small ribosomal subunit protein uS8
45	SA (8)	30S ribosomal protein S9	polymer	130	14872.2	1	UniProt (P0A7X3) Pfam (PF00380)	Escherichia coli K-12	Small ribosomal subunit protein uS9
46	TA (9)	30S ribosomal protein S10	polymer	103	11755.6	1	UniProt (P0A7R5) Pfam (PF00338)	Escherichia coli K-12	Small ribosomal subunit protein uS10,Transcription termination/antitermination protein NusE
47	UA (D)	30S ribosomal protein S11	polymer	129	13844.9	1	UniProt (P0A7R9) Pfam (PF00411)	Escherichia coli K-12	Small ribosomal subunit protein uS11
48	VA (E)	30S ribosomal protein S12	polymer	124	13768.2	1	UniProt (P0A7S3) Pfam (PF00164)	Escherichia coli K-12	Small ribosomal subunit protein uS12
49	WA (F)	30S ribosomal protein S13	polymer	118	13096.4	1	UniProt (P0A7S9) Pfam (PF00416)	Escherichia coli K-12	Small ribosomal subunit protein uS13
50	XA (G)	30S ribosomal protein S14	polymer	101	11562.5	1	UniProt (P0AG59) Pfam (PF00253)	Escherichia coli K-12	Small ribosomal subunit protein uS14
51	YA (H)	30S ribosomal protein S15	polymer	89	10290.8	1	UniProt (P0ADZ4) Pfam (PF00312)	Escherichia coli K-12	Small ribosomal subunit protein uS15
52	ZA (I)	30S ribosomal protein S16	polymer	82	9207.6	1	UniProt (P0A7T3) Pfam (PF00886)	Escherichia coli K-12	Small ribosomal subunit protein bS16
53	AB (J)	30S ribosomal protein S17	polymer	84	9694.5	1	UniProt (P0AG63) Pfam (PF00366)	Escherichia coli K-12	Small ribosomal subunit protein uS17
54	BB (K)	30S ribosomal protein S18	polymer	75	9006.5	1	UniProt (P0A7T7) Pfam (PF01084)	Escherichia coli K-12	Small ribosomal subunit protein bS18
55	CB (t)	30S ribosomal protein S19	polymer	92	10586.5	1	UniProt (P0A7U3) Pfam (PF00203)	Escherichia coli K-12	Small ribosomal subunit protein uS19
56	DB (u)	30S ribosomal protein S20	polymer	87	9690.4	1	UniProt (P0A7U7) Pfam (PF01649)	Escherichia coli K-12	Small ribosomal subunit protein bS20
57	EB (v)	30S ribosomal protein S21	polymer	88	10257.9	1		Escherichia coli K-12
58	FB, GB (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
59	HB (A)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
60	IB (A)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

a: 1 - 127 (UniProt: P0AG44)

PDB	External Database	Details
Ala 123	Glu 123	variant

g: 1 - 100 (UniProt: P0ADZ0)

PDB	External Database	Details
Ser 98	Gly 98	variant

j: 1 - 85 (UniProt: P0A7L8)

PDB	External Database	Details
Unk 3	His 3	variant

L: 1 - 70 (UniProt: P0A7M9)

PDB	External Database	Details
Ser 68	Gly 68	variant

4: 4 - 165 (UniProt: P0A7W1)

PDB	External Database	Details
Met 4	Ile 4	variant

5: 1 - 131 (UniProt: P02358)

PDB	External Database	Details
Ser 101	Pro 101	variant

6: 1 - 156 (UniProt: P02359)

PDB	External Database	Details
His 78	Arg 78	variant

8: 1 - 130 (UniProt: P0A7X3)

PDB	External Database	Details
Asp 3	Glu 3	variant

D: 1 - 129 (UniProt: P0A7R9)

PDB	External Database	Details
Ala 5	Pro 5	variant

F: 1 - 118 (UniProt: P0A7S9)

PDB	External Database	Details
Val 1	Met 1	variant

G: 1 - 101 (UniProt: P0AG59)

PDB	External Database	Details
Ala 40	Asp 40	variant

J: 1 - 84 (UniProt: P0AG63)

PDB	External Database	Details
Ala 2	Thr 2	variant

K: 1 - 75 (UniProt: P0A7T7)

PDB	External Database	Details
Thr 15	Ala 15	variant
Val 19	Gln 19	variant

t: 1 - 92 (UniProt: P0A7U3)

PDB	External Database	Details
Tyr 82	Gly 82	variant
Tyr 83	His 83	variant

u: 1 - 87 (UniProt: P0A7U7)

PDB	External Database	Details
Leu 1	Met 1	variant

Sequence viewer

Contents of the asymmetric unit

Polymers	Number of chains	57
Polymers	Total formula weight	2243733.1
Non-Polymers*	Number of molecules	3
Non-Polymers*	Total formula weight	87.7
All*	Total formula weight	2243820.8

*Water molecules are not included.

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)