7QDP

Crystal structure of FLT3 T343I in complex with the canonical ligand FL

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Fms-related tyrosine kinase 3 ligand	polymer	155	17762.3	4	UniProt (P49771) Pfam (PF02947) In PDB	Homo sapiens (human)	Flt3 ligand,Flt3L,SL cytokine
2	E, F, G, H	Receptor-type tyrosine-protein kinase FLT3	polymer	582	65319.9	4	UniProt (P36888) Pfam (PF00047) In PDB	Homo sapiens (human)	FL cytokine receptor,Fetal liver kinase-2,FLK-2,Fms-like tyrosine kinase 3,FLT-3,Stem cell tyrosine kinase 1,STK-1
3	I	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	J	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	E, F, H	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

A, B, C, D: 1 - 134 (UniProt: P49771)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

E, F, G, H: 1 - 541 (UniProt: P36888)

PDB	External Database	Details
Met 227	Thr 227	variant
Ile 343	Thr 343	engineered mutation
Gly 542	-	expression tag
Ser 543	-	expression tag
Ser 544	-	expression tag
Gly 545	-	expression tag
Leu 546	-	expression tag
Val 547	-	expression tag
Pro 548	-	expression tag
Arg 549	-	expression tag
Gly 550	-	expression tag
Ser 551	-	expression tag
Gly 552	-	expression tag
Gly 553	-	expression tag
Ser 554	-	expression tag
Gly 555	-	expression tag
Gly 556	-	expression tag
Ser 557	-	expression tag
Gly 558	-	expression tag
Leu 559	-	expression tag
Asn 560	-	expression tag
Asp 561	-	expression tag
Ile 562	-	expression tag
Phe 563	-	expression tag
Glu 564	-	expression tag
Ala 565	-	expression tag
Gln 566	-	expression tag
Lys 567	-	expression tag
Ile 568	-	expression tag
Glu 569	-	expression tag
Trp 570	-	expression tag
His 571	-	expression tag
Glu 572	-	expression tag
Gly 573	-	expression tag
Arg 574	-	expression tag
Thr 575	-	expression tag
Lys 576	-	expression tag
His 577	-	expression tag
His 578	-	expression tag
His 579	-	expression tag
His 580	-	expression tag
His 581	-	expression tag
His 582	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	332328.8
Branched	Number of molecules	2
	Total formula weight	1010.9
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	334224.5

*Water molecules are not included.