7QD4

Cryo-EM structure of Tn4430 TnpA transposase from Tn3 family in complex with 100 bp long transposon end DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, D)	Transposase for transposon Tn4430	polymer	1014	116992.1	2	UniProt (P10021)	Bacillus thuringiensis
2	B, D (B, E)	IR100 DNA substrate, none transferred strand	polymer	100	30848.8	2		Bacillus thuringiensis
3	C, E (C, F)	IR100 DNA substrate, transferred strand	polymer	100	30851.7	2		Bacillus thuringiensis

Sequence modifications

A, D: 1 - 987 (UniProt: P10021)

PDB	External Database	Details
His 30	Arg 30	variant
Gln 55	Arg 55	variant
Ala 81	Thr 81	variant
Gln 83	Arg 83	variant
Gln 85	Arg 85	variant
Met 153	Thr 153	variant
Ile 889	Thr 889	variant
Arg 911	Ser 911	engineered mutation
Pro 988	-	expression tag
Ile 989	-	expression tag
Arg 990	-	expression tag
Ser 991	-	expression tag
Phe 992	-	expression tag
Leu 993	-	expression tag
Glu 994	-	expression tag
Gln 995	-	expression tag
Lys 996	-	expression tag
Leu 997	-	expression tag
Ile 998	-	expression tag
Ser 999	-	expression tag
Glu 1000	-	expression tag
Glu 1001	-	expression tag
Asp 1002	-	expression tag
Leu 1003	-	expression tag
Asn 1004	-	expression tag
Ser 1005	-	expression tag
Ala 1006	-	expression tag
Val 1007	-	expression tag
Asp 1008	-	expression tag
His 1009	-	expression tag
His 1010	-	expression tag
His 1011	-	expression tag
His 1012	-	expression tag
His 1013	-	expression tag
His 1014	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	357385.1
All*	Total formula weight	357385.1

*Water molecules are not included.