7QCD
CryoEM structure of the Smc5/6-holocomplex (composite structure)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Structural maintenance of chromosomes protein 5 | polymer | 1093 | 126236.2 | 1 | UniProt (Q08204) Pfam (PF02463) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 2 | B (B) | Structural maintenance of chromosomes protein 6 | polymer | 1114 | 128198.7 | 1 | UniProt (Q12749) Pfam (PF13476) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | DNA repair protein RHC18,Rad18 homolog |
| 3 | C (C) | E3 SUMO-protein ligase MMS21 | polymer | 281 | 31817.8 | 1 | UniProt (P38632) Pfam (PF22326) Pfam (PF11789) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | E3 SUMO-protein transferase MMS21,Methyl methanesulfonate-sensitivity protein 21,Non-structural maintenance of chromosome element 2,Non-SMC element 2 |
| 4 | D (D) | Non-structural maintenance of chromosomes element 1 | polymer | 358 | 40943.1 | 1 | UniProt (Q07913) Pfam (PF07574) Pfam (PF08746) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Non-SMC element 1 |
| 5 | E (E) | Non-structural maintenance of chromosome element 3 | polymer | 303 | 34005.5 | 1 | UniProt (Q05541) Pfam (PF01454) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Non-SMC element 3 |
| 6 | F (F) | Non-structural maintenance of chromosome element 4 | polymer | 715 | 81441.1 | 1 | UniProt (P43124) Pfam (PF15412) Pfam (PF08743) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Non-SMC element 4,Protein QRI2 |
| 7 | G, H, I (C, D) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
A: 1 - 1093 (UniProt: Q08204)
B: 1 - 1114 (UniProt: Q12749)
C: 2 - 267 (UniProt: P38632)
D: 1 - 336 (UniProt: Q07913)
F: 1 - 402 (UniProt: P43124)
| PDB | External Database | Details |
|---|---|---|
| Gln 1015 | Glu 1015 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gln 1048 | Glu 1048 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Tyr -10 | - | expression tag |
| Pro -9 | - | expression tag |
| Tyr -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Val -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -21 | - | initiating methionine |
| Gly -20 | - | expression tag |
| Ser -19 | - | expression tag |
| Ser -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Arg -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 403 | - | expression tag |
| Ser 404 | - | expression tag |
| Ser 405 | - | expression tag |
| Gly 406 | - | expression tag |
| Met 407 | - | expression tag |
| Ala 408 | - | expression tag |
| Glu 409 | - | expression tag |
| Ile 410 | - | expression tag |
| Gly 411 | - | expression tag |
| Thr 412 | - | expression tag |
| Gly 413 | - | expression tag |
| Phe 414 | - | expression tag |
| Pro 415 | - | expression tag |
| Phe 416 | - | expression tag |
| Asp 417 | - | expression tag |
| Pro 418 | - | expression tag |
| His 419 | - | expression tag |
| Tyr 420 | - | expression tag |
| Val 421 | - | expression tag |
| Glu 422 | - | expression tag |
| Val 423 | - | expression tag |
| Leu 424 | - | expression tag |
| Gly 425 | - | expression tag |
| Glu 426 | - | expression tag |
| Arg 427 | - | expression tag |
| Met 428 | - | expression tag |
| His 429 | - | expression tag |
| Tyr 430 | - | expression tag |
| Val 431 | - | expression tag |
| Asp 432 | - | expression tag |
| Val 433 | - | expression tag |
| Gly 434 | - | expression tag |
| Pro 435 | - | expression tag |
| Arg 436 | - | expression tag |
| Asp 437 | - | expression tag |
| Gly 438 | - | expression tag |
| Thr 439 | - | expression tag |
| Pro 440 | - | expression tag |
| Val 441 | - | expression tag |
| Leu 442 | - | expression tag |
| Phe 443 | - | expression tag |
| Leu 444 | - | expression tag |
| His 445 | - | expression tag |
| Gly 446 | - | expression tag |
| Asn 447 | - | expression tag |
| Pro 448 | - | expression tag |
| Thr 449 | - | expression tag |
| Ser 450 | - | expression tag |
| Ser 451 | - | expression tag |
| Tyr 452 | - | expression tag |
| Val 453 | - | expression tag |
| Trp 454 | - | expression tag |
| Arg 455 | - | expression tag |
| Asn 456 | - | expression tag |
| Ile 457 | - | expression tag |
| Ile 458 | - | expression tag |
| Pro 459 | - | expression tag |
| His 460 | - | expression tag |
| Val 461 | - | expression tag |
| Ala 462 | - | expression tag |
| Pro 463 | - | expression tag |
| Thr 464 | - | expression tag |
| His 465 | - | expression tag |
| Arg 466 | - | expression tag |
| Cys 467 | - | expression tag |
| Ile 468 | - | expression tag |
| Ala 469 | - | expression tag |
| Pro 470 | - | expression tag |
| Asp 471 | - | expression tag |
| Leu 472 | - | expression tag |
| Ile 473 | - | expression tag |
| Gly 474 | - | expression tag |
| Met 475 | - | expression tag |
| Gly 476 | - | expression tag |
| Lys 477 | - | expression tag |
| Ser 478 | - | expression tag |
| Asp 479 | - | expression tag |
| Lys 480 | - | expression tag |
| Pro 481 | - | expression tag |
| Asp 482 | - | expression tag |
| Leu 483 | - | expression tag |
| Gly 484 | - | expression tag |
| Tyr 485 | - | expression tag |
| Phe 486 | - | expression tag |
| Phe 487 | - | expression tag |
| Asp 488 | - | expression tag |
| Asp 489 | - | expression tag |
| His 490 | - | expression tag |
| Val 491 | - | expression tag |
| Arg 492 | - | expression tag |
| Phe 493 | - | expression tag |
| Met 494 | - | expression tag |
| Asp 495 | - | expression tag |
| Ala 496 | - | expression tag |
| Phe 497 | - | expression tag |
| Ile 498 | - | expression tag |
| Glu 499 | - | expression tag |
| Ala 500 | - | expression tag |
| Leu 501 | - | expression tag |
| Gly 502 | - | expression tag |
| Leu 503 | - | expression tag |
| Glu 504 | - | expression tag |
| Glu 505 | - | expression tag |
| Val 506 | - | expression tag |
| Val 507 | - | expression tag |
| Leu 508 | - | expression tag |
| Val 509 | - | expression tag |
| Ile 510 | - | expression tag |
| His 511 | - | expression tag |
| Asp 512 | - | expression tag |
| Trp 513 | - | expression tag |
| Gly 514 | - | expression tag |
| Ser 515 | - | expression tag |
| Ala 516 | - | expression tag |
| Leu 517 | - | expression tag |
| Gly 518 | - | expression tag |
| Phe 519 | - | expression tag |
| His 520 | - | expression tag |
| Trp 521 | - | expression tag |
| Ala 522 | - | expression tag |
| Lys 523 | - | expression tag |
| Arg 524 | - | expression tag |
| Asn 525 | - | expression tag |
| Pro 526 | - | expression tag |
| Glu 527 | - | expression tag |
| Arg 528 | - | expression tag |
| Val 529 | - | expression tag |
| Lys 530 | - | expression tag |
| Gly 531 | - | expression tag |
| Ile 532 | - | expression tag |
| Ala 533 | - | expression tag |
| Phe 534 | - | expression tag |
| Met 535 | - | expression tag |
| Glu 536 | - | expression tag |
| Phe 537 | - | expression tag |
| Ile 538 | - | expression tag |
| Arg 539 | - | expression tag |
| Pro 540 | - | expression tag |
| Ile 541 | - | expression tag |
| Pro 542 | - | expression tag |
| Thr 543 | - | expression tag |
| Trp 544 | - | expression tag |
| Asp 545 | - | expression tag |
| Glu 546 | - | expression tag |
| Trp 547 | - | expression tag |
| Pro 548 | - | expression tag |
| Glu 549 | - | expression tag |
| Phe 550 | - | expression tag |
| Ala 551 | - | expression tag |
| Arg 552 | - | expression tag |
| Glu 553 | - | expression tag |
| Thr 554 | - | expression tag |
| Phe 555 | - | expression tag |
| Gln 556 | - | expression tag |
| Ala 557 | - | expression tag |
| Phe 558 | - | expression tag |
| Arg 559 | - | expression tag |
| Thr 560 | - | expression tag |
| Thr 561 | - | expression tag |
| Asp 562 | - | expression tag |
| Val 563 | - | expression tag |
| Gly 564 | - | expression tag |
| Arg 565 | - | expression tag |
| Lys 566 | - | expression tag |
| Leu 567 | - | expression tag |
| Ile 568 | - | expression tag |
| Ile 569 | - | expression tag |
| Asp 570 | - | expression tag |
| Gln 571 | - | expression tag |
| Asn 572 | - | expression tag |
| Val 573 | - | expression tag |
| Phe 574 | - | expression tag |
| Ile 575 | - | expression tag |
| Glu 576 | - | expression tag |
| Gly 577 | - | expression tag |
| Thr 578 | - | expression tag |
| Leu 579 | - | expression tag |
| Pro 580 | - | expression tag |
| Met 581 | - | expression tag |
| Gly 582 | - | expression tag |
| Val 583 | - | expression tag |
| Val 584 | - | expression tag |
| Arg 585 | - | expression tag |
| Pro 586 | - | expression tag |
| Leu 587 | - | expression tag |
| Thr 588 | - | expression tag |
| Glu 589 | - | expression tag |
| Val 590 | - | expression tag |
| Glu 591 | - | expression tag |
| Met 592 | - | expression tag |
| Asp 593 | - | expression tag |
| His 594 | - | expression tag |
| Tyr 595 | - | expression tag |
| Arg 596 | - | expression tag |
| Glu 597 | - | expression tag |
| Pro 598 | - | expression tag |
| Phe 599 | - | expression tag |
| Leu 600 | - | expression tag |
| Asn 601 | - | expression tag |
| Pro 602 | - | expression tag |
| Val 603 | - | expression tag |
| Asp 604 | - | expression tag |
| Arg 605 | - | expression tag |
| Glu 606 | - | expression tag |
| Pro 607 | - | expression tag |
| Leu 608 | - | expression tag |
| Trp 609 | - | expression tag |
| Arg 610 | - | expression tag |
| Phe 611 | - | expression tag |
| Pro 612 | - | expression tag |
| Asn 613 | - | expression tag |
| Glu 614 | - | expression tag |
| Leu 615 | - | expression tag |
| Pro 616 | - | expression tag |
| Ile 617 | - | expression tag |
| Ala 618 | - | expression tag |
| Gly 619 | - | expression tag |
| Glu 620 | - | expression tag |
| Pro 621 | - | expression tag |
| Ala 622 | - | expression tag |
| Asn 623 | - | expression tag |
| Ile 624 | - | expression tag |
| Val 625 | - | expression tag |
| Ala 626 | - | expression tag |
| Leu 627 | - | expression tag |
| Val 628 | - | expression tag |
| Glu 629 | - | expression tag |
| Glu 630 | - | expression tag |
| Tyr 631 | - | expression tag |
| Met 632 | - | expression tag |
| Asp 633 | - | expression tag |
| Trp 634 | - | expression tag |
| Leu 635 | - | expression tag |
| His 636 | - | expression tag |
| Gln 637 | - | expression tag |
| Ser 638 | - | expression tag |
| Pro 639 | - | expression tag |
| Val 640 | - | expression tag |
| Pro 641 | - | expression tag |
| Lys 642 | - | expression tag |
| Leu 643 | - | expression tag |
| Leu 644 | - | expression tag |
| Phe 645 | - | expression tag |
| Trp 646 | - | expression tag |
| Gly 647 | - | expression tag |
| Thr 648 | - | expression tag |
| Pro 649 | - | expression tag |
| Gly 650 | - | expression tag |
| Val 651 | - | expression tag |
| Leu 652 | - | expression tag |
| Ile 653 | - | expression tag |
| Pro 654 | - | expression tag |
| Pro 655 | - | expression tag |
| Ala 656 | - | expression tag |
| Glu 657 | - | expression tag |
| Ala 658 | - | expression tag |
| Ala 659 | - | expression tag |
| Arg 660 | - | expression tag |
| Leu 661 | - | expression tag |
| Ala 662 | - | expression tag |
| Lys 663 | - | expression tag |
| Ser 664 | - | expression tag |
| Leu 665 | - | expression tag |
| Pro 666 | - | expression tag |
| Asn 667 | - | expression tag |
| Cys 668 | - | expression tag |
| Lys 669 | - | expression tag |
| Ala 670 | - | expression tag |
| Val 671 | - | expression tag |
| Asp 672 | - | expression tag |
| Ile 673 | - | expression tag |
| Gly 674 | - | expression tag |
| Pro 675 | - | expression tag |
| Gly 676 | - | expression tag |
| Leu 677 | - | expression tag |
| Asn 678 | - | expression tag |
| Leu 679 | - | expression tag |
| Leu 680 | - | expression tag |
| Gln 681 | - | expression tag |
| Glu 682 | - | expression tag |
| Asp 683 | - | expression tag |
| Asn 684 | - | expression tag |
| Pro 685 | - | expression tag |
| Asp 686 | - | expression tag |
| Leu 687 | - | expression tag |
| Ile 688 | - | expression tag |
| Gly 689 | - | expression tag |
| Ser 690 | - | expression tag |
| Glu 691 | - | expression tag |
| Ile 692 | - | expression tag |
| Ala 693 | - | expression tag |
| Arg 694 | - | expression tag |
| Trp 695 | - | expression tag |
| Leu 696 | - | expression tag |
| Ser 697 | - | expression tag |
| Thr 698 | - | expression tag |
| Leu 699 | - | expression tag |
| Glu 700 | - | expression tag |
| Ile 701 | - | expression tag |
| Ser 702 | - | expression tag |
| Gly 703 | - | expression tag |
| Gly 704 | - | expression tag |
| Ser 705 | - | expression tag |
| Glu 706 | - | expression tag |
| Gln 707 | - | expression tag |
| Lys 708 | - | expression tag |
| Leu 709 | - | expression tag |
| Ile 710 | - | expression tag |
| Ser 711 | - | expression tag |
| Glu 712 | - | expression tag |
| Glu 713 | - | expression tag |
| Asp 714 | - | expression tag |
| Leu 715 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 442642.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 442838.7 |
