7QBA

CryoEM structure of the ABC transporter NosDFY complexed with nitrous oxide reductase NosZ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable ABC transporter binding protein NosD	polymer	436	48258.5	1	UniProt (P19843) Pfam (PF05048)	Pseudomonas stutzeri (Pseudomonas perfectomarina)
2	B, C (B, C)	Probable ABC transporter ATP-binding protein NosF	polymer	313	34518.4	2	UniProt (P19844) Pfam (PF00005)	Pseudomonas stutzeri (Pseudomonas perfectomarina)
3	D, E (D, E)	Probable ABC transporter permease protein NosY	polymer	276	29449.2	2	UniProt (P19845) Pfam (PF12679)	Pseudomonas stutzeri (Pseudomonas perfectomarina)
4	F, G (H, I)	Nitrous-oxide reductase	polymer	646	71958.6	2	UniProt (P19573) Pfam (PF18793) Pfam (PF18764) Pfam (PF00116)	Pseudomonas stutzeri	N(2)OR,N2O reductase
5	H, J (B, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	I, K (B, C)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
7	L, M (H, I)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)

Sequence modifications

B, C: 2 - 308 (UniProt: P19844)

PDB	External Database	Details
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gln 154	Glu 154	conflict

H, I: 1 - 638 (UniProt: P19573)

PDB	External Database	Details
Trp 639	-	expression tag
Ser 640	-	expression tag
His 641	-	expression tag
Pro 642	-	expression tag
Gln 643	-	expression tag
Phe 644	-	expression tag
Glu 645	-	expression tag
Lys 646	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	320110.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1143.1
All*	Total formula weight	321253.9

*Water molecules are not included.