7Q21

III2-IV2 respiratory supercomplex from Corynebacterium glutamicum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, K (Y, y)	Co-purified unknown transmembrane helices built as polyALA (AscD)	polymer	96	8188.1	2		Corynebacterium glutamicum ATCC 13032
2	B, L (E, e)	Cytochrome c oxidase subunit 3	polymer	205	22457.2	2	UniProt (Q9AEL8) Pfam (PF00510)	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	Cytochrome aa3 subunit 3,Cytochrome c oxidase polypeptide III
3	C, M (H, h)	Uncharacterized membrane protein Cgl2017/cg2211	polymer	147	16385.6	2	UniProt (Q8NP09) Pfam (PF10939)	Corynebacterium glutamicum ATCC 13032	P20
4	D, N (F, f)	Cytochrome c oxidase polypeptide 4	polymer	143	15557.3	2	UniProt (Q8NNK3) Pfam (PF12270)	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	Cytochrome aa3 subunit 4,Cytochrome c oxidase polypeptide IV
5	E, O (G, g)	Cytochrome c oxidase subunit 2	polymer	359	39665.6	2	UniProt (Q8NNK2) Pfam (PF00116)	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	Cytochrome aa3 subunit 2,Cytochrome c oxidase polypeptide II,Oxidase aa(3) subunit 2
6	F, P (D, d)	Cytochrome c oxidase subunit 1	polymer	594	66340.2	2	UniProt (Q79VD7) Pfam (PF00115)	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	Cytochrome aa3 subunit 1,Cytochrome c oxidase polypeptide I
7	G, Q (A, a)	Cytochrome bc1 complex Rieske iron-sulfur subunit	polymer	408	45232.2	2	UniProt (Q79VE8) Pfam (PF19297) Pfam (PF00355)	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	Cytochrome bc1 reductase complex subunit QcrA,Menaquinol--cytochrome c reductase iron-sulfur subunit,Rieske iron-sulfur protein
8	H, R (L, l)	Uncharacterized protein Cgl2664/cg2949	polymer	194	20025.8	2	UniProt (Q8NMB4)	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	P29
9	I, S (B, b)	Cytochrome bc1 complex cytochrome b subunit	polymer	539	59863.7	2	UniProt (Q79VE9) Pfam (PF13631)	Corynebacterium glutamicum ATCC 13032	Cytochrome bc1 reductase complex subunit QcrB,Menaquinol--cytochrome c reductase cytochrome b subunit
10	J, T (C, c)	Cytochrome bc1 complex cytochrome c subunit	polymer	283	29898.8	2	UniProt (Q8NNK5) Pfam (PF00034) Pfam (PF13442)	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	Cytochrome c1,Cytochrome bc1 reductase complex subunit Qcrc,Menaquinol--cytochrome c reductase cytochrome c subunit
11	U (X)	Co-purified unknown peptide built as polyALA (AscE)	polymer	21	1823.3	1		Corynebacterium glutamicum ATCC 13032
12	V (x)	Co-purified unknown peptide built as polyALA (AscE)	polymer	22	1908.4	1		Corynebacterium glutamicum ATCC 13032
13	W, X (V, v)	Actinobacterial supercomplex, subunit C (AscC)	polymer	73	8373.3	2	UniProt (Q8NS61)	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
14	Y, Z (K, k)	Hypothetical membrane protein	polymer	65	6628.9	2	UniProt (Q8NSJ8)	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
15	AA, BC, BD, GD, HA... (E, f, b, F, c...)	CARDIOLIPIN	non-polymer		1464.0	14	Chemie (CDL)
16	AB, BA, DA, GC, JC... (D, E, H, f, g...)	(1R)-2-(dodecanoyloxy)-1-[(phosphonooxy)methyl]ethyl tetradecanoate	non-polymer		564.7	16	Chemie (7PH)
17	AC, CA, CB, CC, DB... (f, E, A, F, b...)	TRIDECANE	non-polymer		184.4	18	Chemie (TRD)
18	EA, PB, XB, ZB (H, B, h)	(2S)-1-(hexadecanoyloxy)propan-2-yl (10S)-10-methyloctadecanoate	non-polymer		595.0	4	Chemie (9XX)
19	FA, YB (H, h)	DOCOSANE	non-polymer		310.6	2	Chemie (TWT)
20	HC, IC, NA, OA, OC... (g, G, d, D)	COPPER (II) ION	non-polymer		63.5	6	Chemie (CU)
21	LC, QA (d, G)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
22	MC, NC, SA, TA (d, D)	HEME-AS	non-polymer		921.0	4	Chemie (HAS)
23	PC, VA (d, D)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
24	BB, XC (A, a)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
25	EB, ED, FD, KB, LB... (A, b, B, a)	MENAQUINONE-9	non-polymer		785.2	6	Chemie (MQ9)
26	AD, GB, VC, YC (l, L, a)	(2R)-2-(hexadecanoyloxy)-3-{[(S)-hydroxy{[(1R,2R,3R,4R,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9S)-9-methyloctadecanoate	non-polymer		853.1	4	Chemie (9YF)
27	HB, LD, MD, UC (L, X, x, d)	PALMITIC ACID	non-polymer		256.4	4	Chemie (PLM)
28	CD, DD, IB, JB (b, B)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)
29	JD, KD, RB, SB (c, C)	HEME C	non-polymer		618.5	4	Chemie (HEC)

Sequence modifications

D, d: 1 - 584 (UniProt: Q79VD7)

PDB	External Database	Details
Ala 585	-	expression tag
Ala 586	-	expression tag
Trp 587	-	expression tag
Ser 588	-	expression tag
His 589	-	expression tag
Pro 590	-	expression tag
Gln 591	-	expression tag
Phe 592	-	expression tag
Glu 593	-	expression tag
Lys 594	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	26
	Total formula weight	680964.8
Non-Polymers*	Number of molecules	92
	Total formula weight	54487.0
All*	Total formula weight	735451.8

*Water molecules are not included.