Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7PUA

Middle assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (CA)	9S rRNA	polymer	621	198441.4	1	GenBank (13740)	Trypanosoma brucei brucei
2	B (CB)	9S rRNA	polymer	3	919.6	1		Trypanosoma brucei brucei
3	C (CC)	uS3m	polymer	74	9070.9	1		Trypanosoma brucei brucei
4	D (CE)	Ribosomal_S5_C domain-containing protein	polymer	435	50147.4	1	UniProt (D0A335)	Trypanosoma brucei brucei
5	E (CF)	bS6m	polymer	160	18861.5	1	UniProt (Q38BW5)	Trypanosoma brucei brucei
6	F (CH)	30S ribosomal protein S8, putative	polymer	282	32766.5	1	UniProt (C9ZZU9)	Trypanosoma brucei brucei
7	G (CI)	uS9m	polymer	443	50201.0	1	UniProt (Q57W62) Pfam (PF00380)	Trypanosoma brucei brucei
8	H (CJ)	LysM domain-containing protein	polymer	817	94321.1	1	UniProt (C9ZPU0)	Trypanosoma brucei brucei
9	I (CK)	uS11m	polymer	326	37925.9	1	UniProt (Q389T7)	Trypanosoma brucei brucei
10	J (CL)	uS12m	polymer	87	10421.6	1		Trypanosoma brucei brucei
11	K (CN)	uS14m	polymer	166	19900.0	1	UniProt (Q580I0)	Trypanosoma brucei brucei
12	L (CO)	uS15m	polymer	429	50401.2	1	UniProt (Q4GZ99)	Trypanosoma brucei brucei
13	M (CP)	bS16m	polymer	188	22077.8	1	UniProt (Q384N9)	Trypanosoma brucei brucei
14	N (CQ)	30S Ribosomal protein S17, putative	polymer	307	35632.7	1	UniProt (C9ZYU9)	Trypanosoma brucei brucei
15	O (CR)	bS18m	polymer	320	36988.7	1	UniProt (D0A3A4)	Trypanosoma brucei brucei
16	P (CS)	uS19m	polymer	244	28364.6	1	UniProt (A0A3L6L621)	Trypanosoma brucei brucei
17	Q (CU)	bS21m	polymer	193	23087.9	1	UniProt (Q580M9)	Trypanosoma brucei brucei
18	R (Ca)	mS22	polymer	602	73064.1	1	UniProt (Q38DR3)	Trypanosoma brucei brucei
19	S (Cb)	mS23	polymer	325	36869.6	1	UniProt (C9ZNU0)	Trypanosoma brucei brucei
20	T (Cd)	mS26	polymer	440	51192.8	1	UniProt (Q38DK6)	Trypanosoma brucei brucei
21	U (Cg)	mS29	polymer	498	57048.8	1	UniProt (Q585C2) Pfam (PF10236)	Trypanosoma brucei brucei
22	V (Ci)	mS33	polymer	181	21244.1	1	UniProt (Q57WW0)	Trypanosoma brucei brucei
23	W (Cj)	mS34	polymer	257	28728.2	1	UniProt (Q57UK0)	Trypanosoma brucei brucei
24	X (Ck)	mS35	polymer	874	98277.6	1	UniProt (Q387C7)	Trypanosoma brucei brucei
25	Y (Cm)	mS37	polymer	215	24757.9	1	UniProt (Q38C96)	Trypanosoma brucei brucei
26	Z (Cn)	mS38	polymer	250	28240.6	1	UniProt (Q57VQ9)	Trypanosoma brucei brucei
27	AA (Cp)	Protein FYV4, mitochondrial	polymer	187	22421.5	1	UniProt (Q389L3) Pfam (PF09597)	Trypanosoma brucei brucei
28	BA (DB)	mS49	polymer	1181	137234.1	1	UniProt (C9ZJE4)	Trypanosoma brucei brucei
29	CA (DC)	mS50	polymer	1165	131719.3	1	UniProt (A0A3L6L3Q2)	Trypanosoma brucei brucei
30	DA (DD)	mS51	polymer	812	93661.4	1	UniProt (D0A752)	Trypanosoma brucei brucei
31	EA (DE)	mS52	polymer	747	84938.2	1	UniProt (Q386Q7)	Trypanosoma brucei brucei
32	FA (DF)	mS53	polymer	666	75338.9	1	UniProt (C9ZXX4)	Trypanosoma brucei brucei
33	GA (DG)	mS54	polymer	631	72046.0	1	UniProt (C9ZNY4)	Trypanosoma brucei brucei
34	HA (DH)	mS55	polymer	581	66676.7	1	UniProt (A0A3L6LGC8)	Trypanosoma brucei brucei
35	IA (DI)	mS56	polymer	407	47306.8	1	UniProt (A0A3L6L6C6)	Trypanosoma brucei brucei
36	JA (DJ)	mS57	polymer	396	45956.3	1	UniProt (Q584U8)	Trypanosoma brucei brucei
37	KA (DK)	mS58	polymer	324	35651.9	1	UniProt (A0A3L6L3U6)	Trypanosoma brucei brucei
38	LA (DL)	mS59	polymer	307	35395.7	1	UniProt (D0A232)	Trypanosoma brucei brucei
39	MA (DO)	mS62	polymer	282	32068.1	1	UniProt (Q383D1)	Trypanosoma brucei brucei
40	NA (DP)	mS63	polymer	274	31922.2	1	UniProt (C9ZXR0)	Trypanosoma brucei brucei
41	OA (DR)	mS65	polymer	270	30617.4	1	UniProt (C9ZPP1)	Trypanosoma brucei brucei
42	PA (DT)	Rhodanese domain-containing protein	polymer	247	30096.1	1	UniProt (Q586G5) Pfam (PF00581)	Trypanosoma brucei brucei
43	QA (DU)	Ubiquitin-like domain-containing protein	polymer	228	26472.8	1	UniProt (C9ZKC1)	Trypanosoma brucei brucei
44	RA (DV)	mS69	polymer	183	21589.7	1	UniProt (Q57UZ6)	Trypanosoma brucei brucei
45	SA (DW)	mS70	polymer	179	21247.4	1	UniProt (D0A8P6)	Trypanosoma brucei brucei
46	TA (DX)	mS71	polymer	169	20093.2	1	UniProt (Q383G5)	Trypanosoma brucei brucei
47	UA (DY)	mS72	polymer	163	19340.4	1	UniProt (Q57YD4)	Trypanosoma brucei brucei
48	VA (DZ)	mS73	polymer	94	11360.9	1	UniProt (Q587C4)	Trypanosoma brucei brucei
49	WA (Da)	mS74	polymer	64	8203.4	1	UniProt (A0A3L6KY67)	Trypanosoma brucei brucei
50	XA (F2)	PPR_long domain-containing protein	polymer	1024	116189.5	1	UniProt (C9ZIL7)	Trypanosoma brucei brucei
51	YA (F3)	mt-SAF3	polymer	966	106621.7	1	UniProt (Q38E61)	Trypanosoma brucei brucei
52	ZA (F5)	mt-SAF5	polymer	754	86949.4	1	UniProt (D0AAF7)	Trypanosoma brucei brucei
53	AB (F6)	DUF4460 domain-containing protein	polymer	676	76781.4	1	UniProt (Q38FQ8) Pfam (PF14687)	Trypanosoma brucei brucei
54	BB (F7)	mt-SAF7	polymer	679	76675.2	1	UniProt (Q57UW6)	Trypanosoma brucei brucei
55	CB (F9)	mt-SAF9	polymer	607	69652.7	1	UniProt (C9ZSL5)	Trypanosoma brucei brucei
56	DB, EB (FM, FN)	mt-SAF21	polymer	370	39638.8	2	UniProt (C9ZJW2)	Trypanosoma brucei brucei
57	FB (FO)	mt-SAF22	polymer	334	39132.6	1	UniProt (Q389F9)	Trypanosoma brucei brucei
58	GB (FP)	mt-SAF23	polymer	349	38240.2	1	UniProt (D0A963)	Trypanosoma brucei brucei
59	HB (FW)	LMWPc domain-containing protein	polymer	263	30538.6	1	UniProt (Q38FI3) Pfam (PF01451)	Trypanosoma brucei brucei
60	IB (FX)	mt-SAF27	polymer	239	26936.7	1	UniProt (Q57YK5) Pfam (PF13812)	Trypanosoma brucei brucei
61	JB (FZ)	mt-SAF29	polymer	178	20048.5	1	UniProt (A0A3L6KWY8)	Trypanosoma brucei brucei
62	KB (Fb)	mt-SAF31	polymer	151	17673.5	1	UniProt (Q581U4)	Trypanosoma brucei brucei
63	LB (Fc)	Acyl carrier protein	polymer	148	16550.8	1	UniProt (Q57WW9) Pfam (PF00550)	Trypanosoma brucei brucei
64	MB (Fd)	DUF4379 domain-containing protein	polymer	143	16291.1	1	UniProt (Q581T4) Pfam (PF14311)	Trypanosoma brucei brucei
65	NB (Ff)	DNA photolyase, putative	polymer	848	95285.3	1	UniProt (D0A9A9)	Trypanosoma brucei brucei
66	OB (Fg)	Acyl transferase-like protein, putative	polymer	550	60722.1	1	UniProt (C9ZYZ4)	Trypanosoma brucei brucei
67	PB (Fh)	mt-SAF37	polymer	318	37009.1	1	UniProt (Q38A63)	Trypanosoma brucei brucei
68	QB (Fi)	mt-SAF38	polymer	629	69516.7	1	UniProt (C9ZNX5)	Trypanosoma brucei brucei
69	RB (IA)	Translation initiation factor IF-2, putative	polymer	787	87114.0	1	UniProt (C9ZSN7)	Trypanosoma brucei brucei
70	SB (IB)	mt-SAF39	polymer	803	89640.0	1	UniProt (D0A0V4)	Trypanosoma brucei brucei
71	TB (U8)	Unk8	polymer	30	2571.2	1		Trypanosoma brucei brucei
72	UB (UC)	UnkC	polymer	9	784.0	1		Trypanosoma brucei brucei
73	VB (UD)	UnkD	polymer	13	1224.5	1		Trypanosoma brucei brucei
74	WB (UF)	UnkF	polymer	36	3081.8	1		Trypanosoma brucei brucei
75	XB (UG)	UnkG	polymer	15	1459.6	1		Trypanosoma brucei brucei
76	YB (UI)	UnkI	polymer	6	528.6	1		Trypanosoma brucei brucei
77	ZB (UK)	UnkK	polymer	24	2060.5	1		Trypanosoma brucei brucei
78	AC, BC, DC (UM, UN, UQ)	Unk	polymer	8	698.9	3		Trypanosoma brucei brucei
79	CC (UP)	UnkP	polymer	32	2741.4	1		Trypanosoma brucei brucei
80	EC (Ua)	Unka	polymer	10	869.1	1		Trypanosoma brucei brucei
81	FC (Ug)	Unkg	polymer	271	23081.3	1		Trypanosoma brucei brucei
82	GC, HC, IC, JC, KC... (CA, CQ, Cg, IA)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG) PubChem (888)
83	LC (Cg)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
84	NC, SC (FW, IA)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4) PubChem (1061)
85	OC (Fc)	S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE	non-polymer		496.6	1	Chemie (PM8)
86	PC (Fd)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
87	QC (Ff)	DIHYDROFLAVINE-ADENINE DINUCLEOTIDE	non-polymer		787.6	1	Chemie (FDA) PubChem (705) PubChem (439491) PubChem (446013) PubChem (9940558)
88	RC (IA)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
89	UC, VC (Cg, IA)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

CA: 1 - 613 (GenBank: 13740)

PDB	External Database	Details
U 614	-	expression tag
U 615	-	expression tag
U 616	-	expression tag
U 617	-	expression tag
U 618	-	expression tag
U 619	-	expression tag
U 620	-	expression tag
U 621	-	expression tag

CI: 1 - 443 (UniProt: Q57W62)

PDB	External Database	Details
Ala 370	Val 370	conflict

CJ: 1 - 817 (UniProt: C9ZPU0)

PDB	External Database	Details
Ser 488	Asn 488	conflict

CK: 1 - 326 (UniProt: Q389T7)

PDB	External Database	Details
Arg 3	Gln 3	conflict
Unk 138	Ile 138	conflict

CS: -71 - 172 (UniProt: A0A3L6L621)

PDB	External Database	Details
Ile -11	Asn 61	conflict
Val -10	Cys 62	conflict
Tyr -9	Leu 63	conflict
Phe -8	Leu 64	conflict
His -7	Pro 65	conflict
Cys -6	Leu 66	conflict
Cys -5	Leu 67	conflict
Thr -4	Tyr 68	conflict
Arg -3	Glu 69	conflict
Lys -2	Glu 70	conflict
-	Val 72	deletion
-	Arg 73	deletion

Cb: 1 - 311 (UniProt: C9ZNU0)

PDB	External Database	Details
Gly 302	Arg 302	conflict
Unk 312	-	expression tag
Cys 313	-	expression tag
Ser 314	-	expression tag
Arg 315	-	expression tag
Asp 316	-	expression tag
Gly 317	-	expression tag
Phe 318	-	expression tag
Ala 319	-	expression tag
Leu 320	-	expression tag
Met 321	-	expression tag
Lys 322	-	expression tag
Ala 323	-	expression tag
Asn 324	-	expression tag
Lys 325	-	expression tag

Cd: 1 - 440 (UniProt: Q38DK6)

PDB	External Database	Details
Unk 299	Gly 299	conflict
Unk 364	Gly 364	conflict

Cg: 1 - 497 (UniProt: Q585C2)

PDB	External Database	Details
Val 181	Ala 181	conflict
Arg 498	-	expression tag

Ck: 1 - 874 (UniProt: Q387C7)

PDB	External Database	Details
Ser 107	Leu 107	conflict
Phe 144	Leu 144	conflict
Tyr 253	Phe 253	conflict
Glu 339	Val 339	conflict
Gly 815	Arg 815	conflict
Gly 871	Glu 871	conflict

DB: 1 - 1181 (UniProt: C9ZJE4)

PDB	External Database	Details
Ile 359	Thr 359	conflict

DD: 18 - 812 (UniProt: D0A752)

PDB	External Database	Details
Unk 1	-	expression tag
Unk 2	-	expression tag
Unk 3	-	expression tag
Unk 4	-	expression tag
Unk 5	-	expression tag
Unk 6	-	expression tag
Unk 7	-	expression tag
Unk 8	-	expression tag
Unk 9	-	expression tag
Unk 10	-	expression tag
Unk 11	-	expression tag
Unk 12	-	expression tag
Unk 13	-	expression tag
Unk 14	-	expression tag
Unk 15	-	expression tag
Unk 16	-	expression tag
Unk 17	-	expression tag
Unk 26	Arg 26	conflict
Unk 27	Met 27	conflict
Unk 28	Met 28	conflict
Unk 29	Arg 29	conflict
Unk 30	Ala 30	conflict
Unk 31	Gly 31	conflict
Unk 32	Ser 32	conflict
Unk 33	Gly 33	conflict
Unk 34	Tyr 34	conflict
Unk 35	Gln 35	conflict
Unk 36	Gln 36	conflict
Unk 37	Leu 37	conflict
Unk 38	Arg 38	conflict
Unk 39	Arg 39	conflict
Unk 40	Met 40	conflict
Unk 41	Gly 41	conflict
Unk 42	Met 42	conflict
Unk 43	Pro 43	conflict
Unk 44	Met 44	conflict
Unk 45	Gln 45	conflict
Unk 46	Val 46	conflict
Unk 47	Gly 47	conflict
Unk 48	Met 48	conflict
Unk 49	Gly 49	conflict
Unk 50	Trp 50	conflict
Unk 51	Arg 51	conflict
Unk 52	Lys 52	conflict
Unk 53	Val 53	conflict
Unk 54	Asp 54	conflict
Unk 55	Ser 55	conflict
Unk 56	Phe 56	conflict

DE: 1 - 747 (UniProt: Q386Q7)

PDB	External Database	Details
Unk 378	Lys 378	conflict
Unk 384	Thr 384	conflict

DF: 1 - 666 (UniProt: C9ZXX4)

PDB	External Database	Details
Thr 18	Ala 18	conflict
Asn 372	Asp 372	conflict
Asn 406	Ser 406	conflict
Unk 636	Gly 636	conflict
Lys 638	Arg 638	conflict

DH: 1 - 581 (UniProt: A0A3L6LGC8)

PDB	External Database	Details
Gln 75	Lys 75	conflict

DK: 1 - 324 (UniProt: A0A3L6L3U6)

PDB	External Database	Details
Asp 97	Ser 97	conflict

DR: 1 - 270 (UniProt: C9ZPP1)

PDB	External Database	Details
Pro 128	Ser 128	conflict

DV: 1 - 183 (UniProt: Q57UZ6)

PDB	External Database	Details
Ala 163	Thr 163	conflict

DY: 1 - 163 (UniProt: Q57YD4)

PDB	External Database	Details
His 34	Asp 34	conflict

F3: 1 - 966 (UniProt: Q38E61)

PDB	External Database	Details
Thr 44	Ala 44	conflict
Val 190	Ile 190	conflict
Ala 303	Ser 303	conflict
Asp 418	Asn 418	conflict

F6: 1 - 675 (UniProt: Q38FQ8)

PDB	External Database	Details
Arg 285	His 285	conflict
Ile 291	Thr 291	conflict
Ala 602	Val 602	conflict
Cys 676	-	expression tag

F7: 1 - 679 (UniProt: Q57UW6)

PDB	External Database	Details
Ile 36	Thr 36	conflict
Glu 470	Lys 470	conflict
Val 474	Ala 474	conflict

F9: 1 - 608 (UniProt: C9ZSL5)

PDB	External Database	Details
-	Glu 381	deletion

FM, FN: 1 - 370 (UniProt: C9ZJW2)

PDB	External Database	Details
His 326	Arg 326	conflict
Unk 330	Gly 330	conflict

Ff: 1 - 848 (UniProt: D0A9A9)

PDB	External Database	Details
Ala 109	Val 109	conflict
Unk 127	Tyr 127	conflict
Gln 138	Arg 138	conflict

Fg: 1 - 550 (UniProt: C9ZYZ4)

PDB	External Database	Details
Ile 159	Val 159	conflict
Ile 271	Val 271	conflict
Met 363	Arg 363	conflict
Unk 399	Glu 399	conflict
Asp 436	Glu 436	conflict

IB: 1 - 803 (UniProt: D0A0V4)

PDB	External Database	Details
Lys 98	Glu 98	conflict
Ile 206	Met 206	conflict
His 572	Arg 572	conflict
Pro 798	Ala 798	conflict

Sequence viewer

Contents of the asymmetric unit

Polymers	Number of chains	84
Polymers	Total formula weight	3537657.3
Non-Polymers*	Number of molecules	14
Non-Polymers*	Total formula weight	2660.0
All*	Total formula weight	3540317.3

*Water molecules are not included.

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)