7PPO

Structure of SidJ/CaM bound to SdeA in pre-glutamylation state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitinating/deubiquitinating enzyme SdeA	polymer	979	111200.4	1	UniProt (Q5ZTK4) Pfam (PF12252) Pfam (PF19048) In PDB	Legionella pneumophila	Effector protein SdeA, Septation initiation protein
2	C	Calmodulin-dependent glutamylase SidJ	polymer	794	91827.0	1	UniProt (Q5ZTK6) In PDB	Legionella pneumophila
3	B	Calmodulin	polymer	168	19066.0	1	UniProt (P0DP24) Pfam (PF13499) In PDB	Homo sapiens (Human)
4	C	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	B	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)

Sequence modifications

A: 231 - 1190 (UniProt: Q5ZTK4)

PDB	External Database	Details
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag
His 216	-	expression tag
His 217	-	expression tag
Ser 218	-	expression tag
Ala 219	-	expression tag
Gly 220	-	expression tag
Leu 221	-	expression tag
Glu 222	-	expression tag
Val 223	-	expression tag
Leu 224	-	expression tag
Phe 225	-	expression tag
Gln 226	-	expression tag
Gly 227	-	expression tag
Pro 228	-	expression tag
Met 229	-	expression tag
Val 230	-	expression tag

C: 99 - 873 (UniProt: Q5ZTK6)

PDB	External Database	Details
His 81	-	expression tag
His 82	-	expression tag
His 83	-	expression tag
His 84	-	expression tag
His 85	-	expression tag
His 86	-	expression tag
Ser 87	-	expression tag
Ala 88	-	expression tag
Gly 89	-	expression tag
Leu 90	-	expression tag
Glu 91	-	expression tag
Val 92	-	expression tag
Leu 93	-	expression tag
Phe 94	-	expression tag
Gln 95	-	expression tag
Gly 96	-	expression tag
Pro 97	-	expression tag
Met 98	-	expression tag
Thr 138	Ala 138	variant
Val 151	Ile 151	variant
Gln 153	Lys 153	variant
Ile 200	Thr 200	variant
Thr 212	Met 212	variant
Arg 371	Lys 371	variant
Gln 383	Glu 383	variant
Ser 393	Arg 393	variant
Tyr 398	His 398	variant
Gly 433	Asp 433	variant
Leu 447	Gln 447	variant
Thr 448	Ser 448	variant
Met 483	Val 483	variant
Ala 565	Glu 565	engineered mutation
Thr 725	Val 725	variant
Gln 767	Glu 767	variant
Arg 798	Gly 798	variant
Lys 834	Arg 834	variant
Thr 848	Ala 848	variant
Asn 855	Lys 855	variant
Glu 859	Asp 859	variant
Lys 874	-	variant

B: 1 - 149 (UniProt: P0DP24)

PDB	External Database	Details
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	222093.4
Non-Polymers*	Number of molecules	2
	Total formula weight	64.4
All*	Total formula weight	222157.8

*Water molecules are not included.