7PP3

STRUCTURE OF ESTER-HYDROLASE EH7 FROM THE METAGENOME OF MARINE SEDIMENTS AT MILAZZO HARBOR (SICILY, ITALY)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Esterase	polymer	429	47867.2	4	UniProt (A0A2K8JQ66) Pfam (PF00144) In PDB	uncultured bacterium
2	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
3	A, B, C, D	GLYCEROL	non-polymer		92.1	10	Chemie (GOL)
4	C	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5		water	water		18.0	484	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 409 (UniProt: A0A2K8JQ66)

PDB	External Database	Details
Met -18	-	initiating methionine
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag
Ser 1	-	expression tag
Ala 410	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	191468.9
Non-Polymers*	Number of molecules	13
	Total formula weight	1168.6
All*	Total formula weight	192637.6

*Water molecules are not included.