7PMN

S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	DNA replication licensing factor MCM2	polymer	868	98911.5	1	UniProt (P29469) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae (Baker's yeast)	Minichromosome maintenance protein 2
2	B (3)	DNA replication licensing factor MCM3	polymer	1009	111987.6	1	UniProt (P24279) Pfam (PF22207) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae (Baker's yeast)	Minichromosome maintenance protein 3
3	C (4)	DNA replication licensing factor MCM4	polymer	933	105138.4	1	UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128)	Saccharomyces cerevisiae (Baker's yeast)	Cell division control protein 54
4	D (5)	Minichromosome maintenance protein 5	polymer	775	86505.7	1	UniProt (P29496) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933)	Saccharomyces cerevisiae (Baker's yeast)	Cell division control protein 46
5	E (6)	DNA replication licensing factor MCM6	polymer	1017	113110.2	1	UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263)	Saccharomyces cerevisiae (Baker's yeast)	Minichromosome maintenance protein 6
6	F (7)	DNA replication licensing factor MCM7	polymer	845	95049.9	1	UniProt (P38132) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae (Baker's yeast)	Cell division control protein 47,Minichromosome maintenance protein 7
7	G (A)	DNA replication complex GINS protein PSF1	polymer	208	24230.6	1	UniProt (Q12488) Pfam (PF05916)	Saccharomyces cerevisiae (Baker's yeast)	Partner of Sld five 1
8	H (B)	DNA replication complex GINS protein PSF2	polymer	213	25096.8	1	UniProt (P40359) Pfam (PF05916)	Saccharomyces cerevisiae (Baker's yeast)	Partner of Sld five 2
9	I (C)	DNA replication complex GINS protein PSF3	polymer	194	22004.2	1	UniProt (A0A6L1BWJ4)	Saccharomyces cerevisiae (Baker's yeast)
10	J (D)	DNA replication complex GINS protein SLD5	polymer	294	33983.6	1	UniProt (Q03406) Pfam (PF05916) Pfam (PF16922)	Saccharomyces cerevisiae (Baker's yeast)
11	K (E)	Cell division control protein 45,Cell division control protein 45	polymer	657	75154.7	1	UniProt (Q08032) Pfam (PF02724)	Saccharomyces cerevisiae (Baker's yeast)
12	L, M, N (F, G, H)	DNA polymerase alpha-binding protein	polymer	962	108610.1	3	UniProt (Q01454) Pfam (PF12341) Pfam (PF20946)	Saccharomyces cerevisiae (Baker's yeast)	Chromosome replication protein CHL15,Chromosome transmission fidelity protein 4,Protein POB1
13	O (I)	Leading strand template DNA	polymer	115	37738.7	1		Synthetic construct
14	P (J)	Lagging strand template DNA	polymer	122	35259.2	1		Saccharomyces cerevisiae
15	Q (K)	Suppressor of kinetochore protein 1	polymer	194	22357.3	1	UniProt (P52286) Pfam (PF03931) Pfam (PF01466)	Saccharomyces cerevisiae (Baker's yeast)	Centromere DNA-binding protein complex CBF3 subunit D,E3 ubiquitin ligase complex SCF subunit SKP1
16	R (L)	Protein DIA2	polymer	735	85368.8	1	UniProt (Q08496) Pfam (PF00646)	Saccharomyces cerevisiae (Baker's yeast)	Digs into agar protein 2
17	S (Q)	DNA polymerase epsilon catalytic subunit A	polymer	2222	255890.4	1	UniProt (P21951) Pfam (PF03104) Pfam (PF22634) Pfam (PF08490) Pfam (PF22912)	Saccharomyces cerevisiae (Baker's yeast)	3'-5' exodeoxyribonuclease,DNA polymerase II subunit A
18	T (R)	DNA polymerase epsilon subunit B	polymer	689	78425.9	1	UniProt (P24482) Pfam (PF04042)	Saccharomyces cerevisiae (Baker's yeast)	DNA polymerase II subunit 2
19	U (X)	Topoisomerase 1-associated factor 1	polymer	1238	141296.9	1	UniProt (P53840) Pfam (PF04821)	Saccharomyces cerevisiae (Baker's yeast)
20	V (Y)	Chromosome segregation in meiosis protein 3	polymer	319	36588.8	1	UniProt (Q04659) Pfam (PF07962)	Saccharomyces cerevisiae (Baker's yeast)
21	AA, W (5, 2)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
22	BA, X (5, 2)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
23	CA, DA, EA, FA, GA... (5, 6, 7, Q, 2...)	ZINC ION	non-polymer		65.4	7	Chemie (ZN)

Sequence modifications

3: 1 - 971 (UniProt: P24279)

PDB	External Database	Details
Met -37	-	initiating methionine
Lys -36	-	expression tag
Arg -35	-	expression tag
Arg -34	-	expression tag
Trp -33	-	expression tag
Lys -32	-	expression tag
Lys -31	-	expression tag
Asn -30	-	expression tag
Phe -29	-	expression tag
Ile -28	-	expression tag
Ala -27	-	expression tag
Val -26	-	expression tag
Ser -25	-	expression tag
Ala -24	-	expression tag
Ala -23	-	expression tag
Asn -22	-	expression tag
Arg -21	-	expression tag
Phe -20	-	expression tag
Lys -19	-	expression tag
Lys -18	-	expression tag
Ile -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Ala -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Glu -3	-	expression tag
Ala -2	-	expression tag
Pro -1	-	expression tag
Val 0	-	expression tag

E: 1 - 167 (UniProt: Q08032)
E: 167 - 650 (UniProt: Q08032)

PDB	External Database	Details
Asp 167	-	expression tag
Tyr 167	-	expression tag
Lys 167	-	expression tag
Asp 167	-	expression tag
Asp 167	-	expression tag
Asp 167	-	expression tag
Gly 167	-	expression tag
Asp 167	-	expression tag
Tyr 167	-	expression tag
Lys 167	-	expression tag
Asp 167	-	expression tag
Asp 167	-	expression tag

F, G, H: 1 - 927 (UniProt: Q01454)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

L: 1 - 732 (UniProt: Q08496)

PDB	External Database	Details
Gly -2	-	expression tag
Ala -1	-	expression tag
Gly 0	-	expression tag

Q: 1 - 2222 (UniProt: P21951)

PDB	External Database	Details
Ala 290	Asp 290	variant
Ala 292	Glu 292	variant

Y: 1 - 317 (UniProt: Q04659)

PDB	External Database	Details
Gly -1	-	expression tag
Glu 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	22
	Total formula weight	1809929.5
Non-Polymers*	Number of molecules	11
	Total formula weight	1518.9
All*	Total formula weight	1811448.4

*Water molecules are not included.