7PHQ

Structure of homo-dimeric Staphylococcus capitis divalent metal ion transporter (DMT) by NabFab-fiducial assisted cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	H, A	NabFab HC	polymer	239	25684.5	2		synthetic construct
2	L, C	NabFab LC	polymer	215	23258.8	2		synthetic construct
3	B, D	Divalent metal cation transporter MntH	polymer	427	47054.5	2	UniProt (A0A4U9TNH6) Pfam (PF01566) In PDB	Staphylococcus capitis
4	N, E	DMT-Nb16_4	polymer	130	14352.8	2		synthetic construct
5	K, F	Anti-Fab nanobody	polymer	123	13390.6	2		Lama glama
6		water	water		18.0	32	Chemie (HOH)

Sequence modifications

B, D: 43 - 448 (UniProt: A0A4U9TNH6)

PDB	External Database	Details
Met 41	-	initiating methionine
Ser 42	-	expression tag
Ala 449	-	expression tag
Leu 450	-	expression tag
Glu 451	-	expression tag
Val 452	-	expression tag
Leu 453	-	expression tag
Phe 454	-	expression tag
Gln 455	-	expression tag
Gly 456	-	expression tag
Pro 457	-	expression tag
His 458	-	expression tag
His 459	-	expression tag
His 460	-	expression tag
His 461	-	expression tag
His 462	-	expression tag
His 463	-	expression tag
His 464	-	expression tag
His 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	247482.5
All*	Total formula weight	247482.5

*Water molecules are not included.