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Structure of Multidrug and Toxin Compound Extrusion (MATE) transporter NorM by NabFab-fiducial assisted cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Multidrug resistance protein NorM	polymer	484	52462.1	1	UniProt (D7H904) Pfam (PF01554) UniProt (by SIFTS) (Q9KRU4) In PDB	Vibrio cholerae RC385	Na(+)/drug antiporter
2	H	NabFab HC	polymer	239	25684.5	1		synthetic construct
3	L	NabFab LC	polymer	215	23258.8	1		synthetic construct
4	N	NorM-Nb17_4	polymer	132	14357.0	1		synthetic construct
5	K	Anti-Fab nanobody	polymer	123	13390.6	1		Lama glama
6		water	water		18.0	21	Chemie (HOH)

Sequence modifications

A: 0 - 457 (UniProt: D7H904)

PDB	External Database	Details
Met -26	-	initiating methionine
Gly -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Val -9	-	expression tag
Leu -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Pro -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Thr -1	-	expression tag
Thr 401	Ile 405	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	129153.0
All*	Total formula weight	129153.0

*Water molecules are not included.