7PHF

Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-hydroxylase RdmB and 10-decarboxylase TamK

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Carminomycin 4-O-methyltransferase DnrK,Methyltransferase domain-containing protein,Aclacinomycin 10-hydroxylase RdmB	polymer	368	39985.4	4	UniProt (Q06528) UniProt (I2N5E8) UniProt (Q54527)	Streptomyces peucetius	COMT,Anthracycline 4-O-methyltransferase,O-methyltransferase,15-demethoxy-epsilon-rhodomycin 10-hydroxylase,15-demethoxyaclacinomycin T
2	E, F, H, J (A, B, C, D)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	4	Chemie (SAH) PubChem (16760024) PubChem (193) PubChem (25210495) PubChem (13792) PubChem (177678424) PubChem (44361184) PubChem (21125603) PubChem (46936206) PubChem (50989525) PubChem (25203782) PubChem (51380914) PubChem (52224398) PubChem (189149) PubChem (57544532) PubChem (439155) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (52224400) PubChem (5284331) PubChem (6419189) PubChem (118374891) PubChem (118560068) PubChem (71170554) PubChem (13231890) PubChem (164157219) PubChem (126969526) PubChem (129105892) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525)
3	G, I, K (B, C, D)	methyl (1R,2R,4S)-2-ethyl-2,4,5,7-tetrahydroxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracene-1-carboxylate	non-polymer		412.4	3	Chemie (VAK) PubChem (159776) PubChem (271302) PubChem (70505688) PubChem (90473257) PubChem (102358222) PubChem (102239684) PubChem (10047286) PubChem (15558570) PubChem (139587812) PubChem (140419317) PubChem (162958247)
4	L, M, N, O (A, B, C, D)	water	water		18.0	223	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 159 (UniProt: Q06528)

PDB	External Database	Details
Met -9	-	initiating methionine
Ala -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Arg 0	-	expression tag
Ser 1	-	expression tag

A, B, C, D: 160 - 169 (UniProt: I2N5E8)
A, B, C, D: 170 - 285 (UniProt: Q06528)

PDB	External Database	Details
Asn 190	Lys 190	engineered mutation

A, B, C, D: 288 - 302 (UniProt: Q54527)
A, B, C, D: 303 - 336 (UniProt: Q06528)
A, B, C, D: 337 - 349 (UniProt: I2N5E8)
A, B, C, D: 350 - 360 (UniProt: Q06528)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	159941.5
Non-Polymers*	Number of molecules	7
	Total formula weight	2774.8
All*	Total formula weight	162716.3

*Water molecules are not included.