7PHF

Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-hydroxylase RdmB and 10-decarboxylase TamK

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Carminomycin 4-O-methyltransferase DnrK,Methyltransferase domain-containing protein,Aclacinomycin 10-hydroxylase RdmB	polymer	368	39985.4	4	UniProt (Q06528) UniProt (I2N5E8) UniProt (Q54527) Pfam (PF00891)	Streptomyces peucetius	COMT,Anthracycline 4-O-methyltransferase,O-methyltransferase,15-demethoxy-epsilon-rhodomycin 10-hydroxylase,15-demethoxyaclacinomycin T
2	E, F, H, J (A, B, C, D)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	4	Chemie (SAH)
3	G, I, K (B, C, D)	methyl (1R,2R,4S)-2-ethyl-2,4,5,7-tetrahydroxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracene-1-carboxylate	non-polymer		412.4	3	Chemie (VAK)
4	L, M, N, O (A, B, C, D)	water	water		18.0	223	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 159 (UniProt: Q06528)

PDB	External Database	Details
Met -9	-	initiating methionine
Ala -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Arg 0	-	expression tag
Ser 1	-	expression tag

A, B, C, D: 160 - 169 (UniProt: I2N5E8)
A, B, C, D: 170 - 285 (UniProt: Q06528)

PDB	External Database	Details
Asn 190	Lys 190	engineered mutation

A, B, C, D: 288 - 302 (UniProt: Q54527)
A, B, C, D: 303 - 336 (UniProt: Q06528)
A, B, C, D: 337 - 349 (UniProt: I2N5E8)
A, B, C, D: 350 - 360 (UniProt: Q06528)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	159941.5
Non-Polymers*	Number of molecules	7
	Total formula weight	2774.8
All*	Total formula weight	162716.3

*Water molecules are not included.