7PHF
Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-hydroxylase RdmB and 10-decarboxylase TamK
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Carminomycin 4-O-methyltransferase DnrK,Methyltransferase domain-containing protein,Aclacinomycin 10-hydroxylase RdmB | polymer | 368 | 39985.4 | 4 | UniProt (Q06528) UniProt (I2N5E8) UniProt (Q54527) Pfam (PF00891) In PDB | Streptomyces peucetius | COMT,Anthracycline 4-O-methyltransferase,O-methyltransferase,15-demethoxy-epsilon-rhodomycin 10-hydroxylase,15-demethoxyaclacinomycin T |
2 | A, B, C, D | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 4 | Chemie (SAH) | |||
3 | B, C, D | methyl (1R,2R,4S)-2-ethyl-2,4,5,7-tetrahydroxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracene-1-carboxylate | non-polymer | 412.4 | 3 | Chemie (VAK) | |||
4 | water | water | 18.0 | 223 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 159 (UniProt: Q06528)
A, B, C, D: 160 - 169 (UniProt: I2N5E8)
A, B, C, D: 170 - 285 (UniProt: Q06528)
A, B, C, D: 288 - 302 (UniProt: Q54527)
A, B, C, D: 303 - 336 (UniProt: Q06528)
A, B, C, D: 337 - 349 (UniProt: I2N5E8)
A, B, C, D: 350 - 360 (UniProt: Q06528)
PDB | External Database | Details |
---|---|---|
Met -9 | - | initiating methionine |
Ala -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
Arg 0 | - | expression tag |
Ser 1 | - | expression tag |
A, B, C, D: 170 - 285 (UniProt: Q06528)
PDB | External Database | Details |
---|---|---|
Asn 190 | Lys 190 | engineered mutation |
A, B, C, D: 303 - 336 (UniProt: Q06528)
A, B, C, D: 337 - 349 (UniProt: I2N5E8)
A, B, C, D: 350 - 360 (UniProt: Q06528)
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 159941.5 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 2774.8 | |
All* | Total formula weight | 162716.3 |