7PH4
AMP-PNP bound nanodisc reconstituted MsbA with nanobodies, spin-labeled at position T68C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | ATP-dependent lipid A-core flippase | polymer | 593 | 65760.9 | 2 | UniProt (P60752) Pfam (PF00664) Pfam (PF00005) | Escherichia coli (strain K12) | Lipid A export ATP-binding/permease protein MsbA,Lipid flippase |
| 2 | C, D (C, D) | Nanobody Nb_MsbA#1 | polymer | 117 | 12410.9 | 2 | Vicugna pacos | ||
| 3 | E, H (A, B) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) | |||
| 4 | F, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | G, J (A, B) | DODECYL-BETA-D-MALTOSIDE | non-polymer | 510.6 | 2 | Chemie (LMT) | |||
| 6 | K, M (C, D) | (1~{R},4~{R},11~{S},14~{S},19~{Z})-19-[2-[2,5-bis(oxidanylidene)pyrrolidin-1-yl]ethylimino]-7,8,17,18-tetraoxa-1,4,11,14-tetrazatricyclo[12.6.2.2^{4,11}]tetracosane-6,9,16-trione | non-polymer | 524.5 | 2 | Chemie (88T) | |||
| 7 | L, N (C, D) | GADOLINIUM ATOM | non-polymer | 157.3 | 2 | Chemie (GD) | |||
| 8 | O, P (A, B) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 582 (UniProt: P60752)
| PDB | External Database | Details |
|---|---|---|
| Gly -10 | - | expression tag |
| Pro -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Ala -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Gln 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 156343.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 3445.8 | |
| All* | Total formula weight | 159789.3 |
