7PCO
BurG E232Q mutant (holo) in complex with 2R,3R-2,3-dihydroxy-6-methyl-heptanoate (12): Biosynthesis of cyclopropanol rings in bacterial toxins
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Ketol-acid reductoisomerase | polymer | 358 | 38715.7 | 2 | UniProt (Q2T3G7) Pfam (PF07991) Pfam (PF01450) | Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264) | |
| 2 | C, H (A, B) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 2 | Chemie (NAD) | |||
| 3 | D, E, I, J (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 4 | F, G (A, B) | (2R,3R)-6-methyl-2,3-bis(oxidanyl)heptanoic acid | non-polymer | 176.2 | 2 | Chemie (7FI) | |||
| 5 | K (B) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 6 | L (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | M, N (A, B) | water | water | 18.0 | 423 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 353 (UniProt: Q2T3G7)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ser -3 | - | expression tag |
| His -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ser 1 | - | expression tag |
| Gln 232 | Glu 232 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 77431.4 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1891.6 | |
| All* | Total formula weight | 79323.0 |
