7PCO
BurG E232Q mutant (holo) in complex with 2R,3R-2,3-dihydroxy-6-methyl-heptanoate (12): Biosynthesis of cyclopropanol rings in bacterial toxins
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Ketol-acid reductoisomerase | polymer | 358 | 38715.7 | 2 | UniProt (Q2T3G7) Pfam (PF07991) Pfam (PF01450) In PDB | Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264) | |
2 | A, B | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 2 | Chemie (NAD) | |||
3 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
4 | A, B | (2R,3R)-6-methyl-2,3-bis(oxidanyl)heptanoic acid | non-polymer | 176.2 | 2 | Chemie (7FI) | |||
5 | B | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
6 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
7 | water | water | 18.0 | 423 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 353 (UniProt: Q2T3G7)
PDB | External Database | Details |
---|---|---|
Gly -4 | - | expression tag |
Ser -3 | - | expression tag |
His -2 | - | expression tag |
Met -1 | - | expression tag |
Ala 0 | - | expression tag |
Ser 1 | - | expression tag |
Gln 232 | Glu 232 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 77431.4 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 1891.6 | |
All* | Total formula weight | 79323.0 |