7PCA

Functional and structural characterization of redox sensitive superfolder green fluorescent protein and variants

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Green fluorescent protein	polymer	238	26752.2	1	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Water jellyfish Mesonema victoria)
2	B, E (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	C, F (A)	ETHANOL	non-polymer		46.1	2	Chemie (EOH)
4	D, G (A)	FORMAMIDE	non-polymer		45.0	2	Chemie (ARF)
5	H (A)	water	water		18.0	281	Chemie (HOH)

Sequence modifications

A: 1 - 238 (UniProt: P42212)

PDB	External Database	Details
Val 1	Met 1	engineered mutation
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Ser 48	Cys 48	engineered mutation
Leu 64	Phe 64	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Arg 80	Gln 80	engineered mutation
Ser 99	Phe 99	engineered mutation
Thr 105	Asn 105	engineered mutation
Phe 145	Tyr 145	engineered mutation
Cys 147	Ser 147	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Val 171	Ile 171	engineered mutation
Cys 204	Gln 204	engineered mutation
Val 206	Ala 206	engineered mutation
Arg 223	Phe 223	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	26752.2
Non-Polymers*	Number of molecules	6
	Total formula weight	366.4
All*	Total formula weight	27118.6

*Water molecules are not included.